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BNIP3L Recombinant Rabbit Monoclonal Antibody [JE59-98]

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Specification

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Catalog# HA722056

BNIP3L Recombinant Rabbit Monoclonal Antibody [JE59-98]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

BNIP3L Recombinant Rabbit Monoclonal Antibody [JE59-98]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human BNIP3L aa 120-219 / 219.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 24 kDa

Positive Control

293T cell lysate, HeLa cell lysate, MCF7 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, human bone marrow tissue, human kidney tissue, human thyroid cancer tissue, mouse adipose tissue.

Conjugation

unconjugated

Clone Number

JE59-98

RRID

AB_3096839

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:500-1:2,000

Target

Function

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like is a protein that in humans is encoded by the BNIP3L gene. This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression. BNIP3L has been shown to interact with STEAP3.

Background References

1. Wu X et al. BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains. Autophagy. 2021 Aug

2. Choi GE et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. Nat Commun. 2021 Jan

Subcellular Location

Nucleus envelope, Endoplasmic reticulum, Mitochondrion outer membrane, Membrane.

UNIPROT

SwissProt: O60238 Human

SwissProt: Q9Z2F7 Mouse

Entrez Gene: 140923 Rat

Synonyms

Adenovirus E1B19k binding protein B5 antibody

Adenovirus E1B19K-binding protein B5 antibody

BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody

BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody

BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody

BNI3L_HUMAN antibody

BNIP3a antibody

BNIP3H antibody

Expand

Images

  • Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 24 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 1 minute 21 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (HA722056) at 1/2,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 24 kDa
    Observed band size: 37 kDa

    Exposure time: 1 minute 21 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722056) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/1,000 dilution.<br /><br />Lane 1: Neuro-2a cell lysate<br />Lane 2: C2C12 cell lysate<br />Lane 3: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 24 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 5 minutes 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (HA722056) at 1/1,000 dilution.

    Lane 1: Neuro-2a cell lysate
    Lane 2: C2C12 cell lysate
    Lane 3: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 24 kDa
    Observed band size: 37 kDa

    Exposure time: 5 minutes 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-BNIP3L antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BNIP3L antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-BNIP3L antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse adipose tissue with Rabbit anti-BNIP3L antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722056" style="font-weight: bold;text-decoration: underline;">HA722056</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse adipose tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation