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Cathepsin L Recombinant Rabbit Monoclonal Antibody [PSH03-91]

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Specification

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Catalog# HA722063

Cathepsin L Recombinant Rabbit Monoclonal Antibody [PSH03-91]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cathepsin L Recombinant Rabbit Monoclonal Antibody [PSH03-91]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Cathepsin L aa 84-333 / 333.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 38 kDa

Positive Control

A549 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, human kidney tissue lysate, human liver tissue lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, COS-1 cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, A549.

Conjugation

unconjugated

Clone Number

PSH03-91

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:5,000

  • FC

  • 1:1,000

Target

Function

Cathepsin L1 is a protein that in humans is encoded by the CTSL1 gene. The protein is a cysteine cathepsin, a lysosomal cysteine protease that plays a major role in intracellular protein catabolism. Cathepsin L1 is a member of the Peptidase C1 (cathepsin) MEROPS family, which plays an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

Background References

1. Zhao MM et al. Cathepsin L plays a key role in SARS-CoV-2 infection in humans and humanized mice and is a promising target for new drug development. Signal Transduct Target Ther. 2021 Mar

2. Gomes CP et al. Cathepsin L in COVID-19: From Pharmacological Evidences to Genetics. Front Cell Infect Microbiol. 2020 Dec

Subcellular Location

Lysosome, Apical cell membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule, Secreted, extracellular space.

UNIPROT

SwissProt: P07711 Human

SwissProt: P06797 Mouse

SwissProt: P07154 Rat

Synonyms

Cathepsin L antibody

cathepsin L, 1 b antibody

Cathepsin L1 antibody

Cathepsin L1 light chain antibody

CathepsinL antibody

CATL antibody

CATL1_HUMAN antibody

cb15 antibody

CTSL antibody

CTSL1 antibody

Expand

Images

  • Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549 cell lysate (15 µg/Lane)<br />Lane 2: HepG2 cell lysate (15 µg/Lane)<br />Lane 3: HEK-293 cell lysate (15 µg/Lane)<br />Lane 4: MDA-MB-231 cell lysate (15 µg/Lane)<br />Lane 5: Human kidney tissue lysate (30 µg/Lane)<br />Lane 6: Human liver tissue lysate (30 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (15 µg/Lane)<br />Lane 8: PC-12 cell lysate (15 µg/Lane)<br />Lane 9: Mouse kidney tissue lysate (30 µg/Lane)<br />Lane 10: Rat kidney tissue lysate (30 µg/Lane)<br />Lane 11: Mouse liver tissue lysate (30 µg/Lane)<br />Lane 12: Rat liver tissue lysate (30 µg/Lane)<br />Lane 13: COS-1 cell lysate (15 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 20-45 kDa<br /><br />Exposure time: 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution.

    Lane 1: A549 cell lysate (15 µg/Lane)
    Lane 2: HepG2 cell lysate (15 µg/Lane)
    Lane 3: HEK-293 cell lysate (15 µg/Lane)
    Lane 4: MDA-MB-231 cell lysate (15 µg/Lane)
    Lane 5: Human kidney tissue lysate (30 µg/Lane)
    Lane 6: Human liver tissue lysate (30 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (15 µg/Lane)
    Lane 8: PC-12 cell lysate (15 µg/Lane)
    Lane 9: Mouse kidney tissue lysate (30 µg/Lane)
    Lane 10: Rat kidney tissue lysate (30 µg/Lane)
    Lane 11: Mouse liver tissue lysate (30 µg/Lane)
    Lane 12: Rat liver tissue lysate (30 µg/Lane)
    Lane 13: COS-1 cell lysate (15 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 20-45 kDa

    Exposure time: 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722063) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin L antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722063) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cathepsin L antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722063) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Cathepsin L antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722063) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of A549 cells labeling Cathepsin L.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A549 cells labeling Cathepsin L.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722063, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si Cathepsin L cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722063" style="font-weight: bold;text-decoration: underline;">HA722063</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si Cathepsin L cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 20 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722063) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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Cathepsin L Recombinant Rabbit Monoclonal Antibody [PSH03-92]

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Cathepsin L Recombinant Rabbit Monoclonal Antibody [PSH03-92] - BSA and Azide free

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Cathepsin L Recombinant Rabbit Monoclonal Antibody [PSH03-91] - BSA and Azide free

Application: WB,IHC-P,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated