TPOR Recombinant Rabbit Monoclonal Antibody [JE58-00] (HA722068)
Catalog# HA722068
Rabbit Monoclonal to TPOR
Tested for
-
WB
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TPOR on different lysates with Rabbit anti-TPOR antibody (HA722068) at 1/1,000 dilution.
Lane 1: Mouse placenta tissue lysate (40 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (no heat) (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 71 kDa
Observed band size: 80 kDa
Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse placenta tissue lysate (40 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (no heat) (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 71 kDa
Observed band size: 80 kDa
Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.