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Sortilin Recombinant Rabbit Monoclonal Antibody [JE60-00]

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Specification

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Catalog# HA722070

Sortilin Recombinant Rabbit Monoclonal Antibody [JE60-00]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Sortilin Recombinant Rabbit Monoclonal Antibody [JE60-00]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Sortilin aa 601-800 / 831.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 92 kDa

Positive Control

SK-MEL-28 cell lysate, human lung tissue lysate, human kidney tissue lysate, human liver tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, human brain tissue.

Conjugation

unconjugated

Clone Number

JE60-00

RRID

AB_3096726

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Sortilin (SORT1) is a protein that in humans is encoded by the SORT1 gene on chromosome 1. This protein is a type I membrane glycoprotein in the vacuolar protein sorting 10 protein (Vps10p) family of sorting receptors. While it is ubiquitously expressed in many tissues, sortilin is most abundant in the central nervous system. At the cellular level, sortilin functions in protein transport between the Golgi apparatus, endosome, lysosome, and plasma membrane, leading to its involvement in multiple biological processes such as glucose and lipid metabolism as well as neural development and cell death. Moreover, the function and role of sortilin is now emerging in several major human diseases such as hypertension, atherosclerosis, coronary artery disease, Alzheimer’s disease, and cancer. The SORT1 gene also contains one of 27 loci associated with increased risk of coronary artery disease.

Background References

1. Yang W et al. Role of sortilin 1 (SORT1) on lipid metabolism in bovine liver. J Dairy Sci. 2022 Jun

2. Yamamoto T et al. SORT1/LAMP2-mediated extracellular vesicle secretion and cell adhesion are linked to lenalidomide resistance in multiple myeloma. Blood Adv. 2022 Apr

Subcellular Location

Golgi apparatus, Golgi stack membrane, Endosome membrane, Endoplasmic reticulum membrane, Nucleus membrane, Cell membrane, Lysosome membrane.

UNIPROT

SwissProt: Q99523 Human

Synonyms

100 kDa NT receptor antibody

Glycoprotein 95 antibody

Gp 95 antibody

Gp95 antibody

LDLCQ6 antibody

Neurotensin receptor 3 antibody

NT 3 antibody

NT3 antibody

NTR 3 antibody

NTR3 antibody

Expand

Images

  • Western blot analysis of Sortilin on different lysates with Rabbit anti-Sortilin antibody (<a href="/products/HA722070" style="font-weight: bold;text-decoration: underline;">HA722070</a>) at 1/1,000 dilution.<br /><br />Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)<br />Lane 2: Human lung tissue lysate (40 µg/Lane)<br />Lane 3: Human kidney tissue lysate (40 µg/Lane)<br />Lane 4: Human liver tissue lysate (40 µg/Lane)<br />Lane 5: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 6: Human brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 92 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: Lane 1-4: 1 minute 7 seconds; Lane 5-6: 2 minutes 37 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722070" style="font-weight: bold;text-decoration: underline;">HA722070</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Sortilin on different lysates with Rabbit anti-Sortilin antibody (HA722070) at 1/1,000 dilution.

    Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
    Lane 2: Human lung tissue lysate (40 µg/Lane)
    Lane 3: Human kidney tissue lysate (40 µg/Lane)
    Lane 4: Human liver tissue lysate (40 µg/Lane)
    Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 6: Human brain tissue lysate (40 µg/Lane)

    Predicted band size: 92 kDa
    Observed band size: 100 kDa

    Exposure time: Lane 1-4: 1 minute 7 seconds; Lane 5-6: 2 minutes 37 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Sortilin antibody (<a href="/products/HA722070" style="font-weight: bold;text-decoration: underline;">HA722070</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722070" style="font-weight: bold;text-decoration: underline;">HA722070</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Sortilin antibody (HA722070) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722070) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"