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CPT1A Recombinant Rabbit Monoclonal Antibody [PSH04-00]

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Specification

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Catalog# HA722081

CPT1A Recombinant Rabbit Monoclonal Antibody [PSH04-00]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CPT1A Recombinant Rabbit Monoclonal Antibody [PSH04-00]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CPT1A aa 201-773 / 773.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 88 kDa

Positive Control

293T cell lysate, HeLa cell lysate, SK-OV-3 cell lysate, MCF7 cell lysate, HepG2 cell lysate, A549 cell lysate, Human kidney tissue lysate, human kidney tissue, human ovary cancer tissue, mouse brain tissue, mouse hippocampus tissue, mouse kidney tissue.

Conjugation

unconjugated

Clone Number

PSH04-00

RRID

AB_3096550

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500-1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I, CPTI, CAT1, CoA:carnitine acyl transferase (CCAT), or palmitoylCoA transferase I, is a mitochondrial enzyme responsible for the formation of acyl carnitines by catalyzing the transfer of the acyl group of a long-chain fatty acyl-CoA from coenzyme A to l-carnitine. The product is often Palmitoylcarnitine (thus the name), but other fatty acids may also be substrates. It is part of a family of enzymes called carnitine acyltransferases. This "preparation" allows for subsequent movement of the acyl carnitine from the cytosol into the intermembrane space of mitochondria. Three isoforms of CPT1 are currently known: CPT1A, CPT1B, and CPT1C. CPT1 is associated with the outer mitochondrial membrane. This enzyme can be inhibited by malonyl CoA, the first committed intermediate produced during fatty acid synthesis. Its role in fatty acid metabolism makes CPT1 important in many metabolic disorders such as diabetes. Since its crystal structure is not known, its exact mechanism of action remains to be determined.

Background References

1. Schlaepfer IR et al. CPT1A-mediated Fat Oxidation, Mechanisms, and Therapeutic Potential. Endocrinology. 2020 Feb

2. Miguel V et al. Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis. J Clin Invest. 2021 Mar

Subcellular Location

Mitochondrion outer membrane.

UNIPROT

SwissProt: P50416 Human

SwissProt: P97742 Mouse

Synonyms

Carnitine O palmitoyltransferase 1 liver isoform antibody

Carnitine O palmitoyltransferase I antibody

Carnitine O palmitoyltransferase I liver isoform antibody

Carnitine O-palmitoyltransferase 1 antibody

Carnitine O-palmitoyltransferase I antibody

Carnitine palmitoyltransferase 1A (liver) antibody

Carnitine palmitoyltransferase 1A antibody

Carnitine palmitoyltransferase I antibody

Carnitine palmitoyltransferase I liver antibody

CPT 1 antibody

Expand

Images

  • Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: SK-OV-3 cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: HepG2 cell lysate<br />Lane 6: A549 cell lysate<br />Lane 7: Human kidney tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 88 kDa<br />Observed band size: 88 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (HA722081) at 1/2,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: SK-OV-3 cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: A549 cell lysate
    Lane 7: Human kidney tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 88 kDa
    Observed band size: 88 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722081) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (HA722081) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-CPT1A antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-CPT1A antibody (HA722081) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CPT1A antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CPT1A antibody (HA722081) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CPT1A antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CPT1A antibody (HA722081) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CPT1A antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CPT1A antibody (HA722081) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722081) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CPT1A was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722081" style="font-weight: bold;text-decoration: underline;">HA722081</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 4 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    CPT1A was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722081 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722081 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA722081 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA722081 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 4 seconds; ECL: K1801

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Citation