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LIAS Recombinant Rabbit Monoclonal Antibody [PSH04-04]

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Specification

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Catalog# HA722085

LIAS Recombinant Rabbit Monoclonal Antibody [PSH04-04]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

LIAS Recombinant Rabbit Monoclonal Antibody [PSH04-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human LIAS aa 1-372 / 372.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 42 kDa

Positive Control

K-562 cell lysate, LO2 cell lysate, Raji cell lysate, MCF7 cell lysate, Jurkat cell lysate, HepG2 cell lysate, PC-3M cell lysate, C2C12 cell lysate, L6 cell lysate, mouse liver tissue lysate, mouse colon tissue lysate, mouse brain tissue lysate, rat liver tissue lysate, rat testis tissue lysate, C2C12.

Conjugation

unconjugated

Clone Number

PSH04-04

RRID

AB_3096624

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

Lipoic acid synthetase is a protein that in humans is encoded by the LIAS gene. The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Background References

1. Xu G et al. Lias overexpression alleviates pulmonary injury induced by fine particulate matter in mice. Environ Geochem Health. 2023 Aug

2. Li Y et al. Exploring the role of LIAS-related cuproptosis in systemic lupus erythematosus. Lupus. 2023 Dec

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: O43766 Human

SwissProt: Q99M04 Mouse

SwissProt: Q5XIH4 Rat

Synonyms

HUSSY 01 antibody

LAS antibody

LIP 1 antibody

Lip syn antibody

LIP1 antibody

Lipoate synthase antibody

Lipoic acid synthetase antibody

Lipoic acid synthetase mitochondrial antibody

Lipoyl synthase, mitochondrial antibody

LS antibody

Expand

Images

  • Western blot analysis of LIAS on different lysates with Rabbit anti-LIAS antibody (<a href="/products/HA722085" style="font-weight: bold;text-decoration: underline;">HA722085</a>) at 1/2,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: LO2 cell lysate (20 µg/Lane)<br />Lane 3: Raji cell lysate (20 µg/Lane)<br />Lane 4: MCF7 cell lysate (20 µg/Lane)<br />Lane 5: Jurkat cell lysate (20 µg/Lane)<br />Lane 6: HepG2 cell lysate (20 µg/Lane)<br />Lane 7: PC-3M cell lysate (20 µg/Lane)<br />Lane 8: C2C12 cell lysate (20 µg/Lane)<br />Lane 9: L6 cell lysate (20 µg/Lane)<br />Lane 10: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 11: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 12: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 13: Rat liver tissue lysate (40 µg/Lane)<br />Lane 14: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 42 kDa<br />Observed band size: 39 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722085" style="font-weight: bold;text-decoration: underline;">HA722085</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LIAS on different lysates with Rabbit anti-LIAS antibody (HA722085) at 1/2,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: LO2 cell lysate (20 µg/Lane)
    Lane 3: Raji cell lysate (20 µg/Lane)
    Lane 4: MCF7 cell lysate (20 µg/Lane)
    Lane 5: Jurkat cell lysate (20 µg/Lane)
    Lane 6: HepG2 cell lysate (20 µg/Lane)
    Lane 7: PC-3M cell lysate (20 µg/Lane)
    Lane 8: C2C12 cell lysate (20 µg/Lane)
    Lane 9: L6 cell lysate (20 µg/Lane)
    Lane 10: Mouse liver tissue lysate (40 µg/Lane)
    Lane 11: Mouse colon tissue lysate (40 µg/Lane)
    Lane 12: Mouse brain tissue lysate (40 µg/Lane)
    Lane 13: Rat liver tissue lysate (40 µg/Lane)
    Lane 14: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 42 kDa
    Observed band size: 39 kDa

    Exposure time: 42 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of C2C12 cells labeling LIAS with Rabbit anti-LIAS antibody (<a href="/products/HA722085" style="font-weight: bold;text-decoration: underline;">HA722085</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIAS antibody (<a href="/products/HA722085" style="font-weight: bold;text-decoration: underline;">HA722085</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of C2C12 cells labeling LIAS with Rabbit anti-LIAS antibody (HA722085) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIAS antibody (HA722085) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation