Mouse PD1 Recombinant Rabbit Monoclonal Antibody [PSH04-18] - BSA and Azide free (Detector)
Overview
Product Name
Mouse PD1 Recombinant Rabbit Monoclonal Antibody [PSH04-18] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse PD1 aa 25-167 (Q02242).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 31.8 kDa
Positive Control
Recombinant standard Mouse PD1 protein (HA210625).
Conjugation
unconjugated
Clone Number
PSH04-18
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-17] to Mouse PD1 (Capture) (HA722100) and recombinant standard Mouse PD1 protein (HA210625). The reference range value is 12.3-3,000pg/mL.
Target
Function
Programmed cell death protein 1 (PD-1), (CD279 cluster of differentiation 279). PD-1 is a protein encoded in humans by the PDCD1 gene. PD-1 is a cell surface receptor on T cells and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells). PD-1 inhibitors, a new class of drugs that block PD-1, activate the immune system to attack tumors and are used to treat certain types of cancer. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells. PD-1 binds two ligands, PD-L1 and PD-L2.
Background References
1. Wei CY et al. PKCα/ZFP64/CSF1 axis resets the tumor microenvironment and fuels anti-PD1 resistance in hepatocellular carcinoma. J Hepatol. 2022 Jul
2. Lei Q et al. Resistance Mechanisms of Anti-PD1/PDL1 Therapy in Solid Tumors. Front Cell Dev Biol. 2020 Jul
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD279 antibody
CD279 antigen antibody
hPD 1 antibody
hPD l antibody
hPD-1 antibody
hSLE1 antibody
PD 1 antibody
PD-1 antibody
PD1 antibody
PDCD 1 antibody
ExpandImages
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Sandwich ELISA analysis of Mouse PD-1 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722100) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PD-1 protein (HA210625) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722101) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
The concentrations of PD-1 were interpolated from the PD-1 standard curve and corrected for sample dilution. The mean PD-1 concentration was determined to be 141 pg/ml in mouse spleen tissue extract, 231 pg/ml in mouse thymus tissue extract. There was no detectable signal in NIH/3T3 cell extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"