Phospho-BCAR1 (Y410) Recombinant Rabbit Monoclonal Antibody [JE47-97]
Catalog# HA722124
Phospho-BCAR1 (Y410) Recombinant Rabbit Monoclonal Antibody [JE47-97]
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WB
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Human
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unconjugated
Overview
Product Name
Phospho-BCAR1 (Y410) Recombinant Rabbit Monoclonal Antibody [JE47-97]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr410 of Human BCAR1.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 93 kDa
Positive Control
HeLa cell lysate, HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate.
Conjugation
unconjugated
Clone Number
JE47-97
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Breast cancer anti-estrogen resistance protein 1 is a protein that in humans is encoded by the BCAR1 gene. BCAR1 is a ubiquitously expressed adaptor molecule originally identified as the major substrate of v-Src and v-Crk . p130Cas/BCAR1 belongs to the Cas family of adaptor proteins and can act as a docking protein for several signalling partners. Due to its ability to associate with multiple signaling partners, p130Cas/BCAR1 contributes to the regulation to a variety of signaling pathways leading to cell adhesion, migration, invasion, apoptosis, hypoxia and mechanical forces. p130Cas/BCAR1 plays a role in cell transformation and cancer progression and alterations of p130Cas/BCAR1 expression and the resulting activation of selective signalling are determinants for the occurrence of different types of human tumors.
Background References
1. Wang R et al. FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling. Int J Biol Sci. 2023 Oct
2. Centonze G et al. p130Cas/BCAR1 and p140Cap/SRCIN1 Adaptors: The Yin Yang in Breast Cancer? Front Cell Dev Biol. 2021 Oct
Subcellular Location
Cell junction, focal adhesion, Cytoplasm, Cell projection, axon.
UNIPROT
Synonyms
BCAR 1 antibody
Bcar1 antibody
BCAR1_HUMAN antibody
Breast cancer anti estrogen resistance 1 antibody
Breast cancer anti estrogen resistance 1 protein antibody
Breast cancer anti-estrogen resistance protein 1 antibody
CAS antibody
Cas scaffolding protein family member 1 antibody
CAS1 antibody
Cass1 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-BCAR1 (Y410) on different lysates with Rabbit anti-Phospho-BCAR1 (Y410) antibody (HA722124) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 130 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722124) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"