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SMCHD1 Recombinant Rabbit Monoclonal Antibody [JE34-29]

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Specification

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Catalog# HA722141

SMCHD1 Recombinant Rabbit Monoclonal Antibody [JE34-29]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

SMCHD1 Recombinant Rabbit Monoclonal Antibody [JE34-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant fragment within Human SMCHD1 aa 1800-2000.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 226 kDa

Positive Control

293T cell lysate, HepG2 cell lysate, HeLa cell lysate, U-87 MG cell lysate, U-2 OS cell lysate, human breast cancer tissue, human testis tissue, human tonsil tissue.

Conjugation

unconjugated

Clone Number

JE34-29

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Structural Maintenance of Chromosomes flexible Hinge Domain Containing 1 (SMCHD1) is a protein that in humans is encoded by the SMCHD1 gene. Mutations in SMCHD1 are causative for development of facioscapulohumeral muscular dystrophy type 2 (FSHD2) and Bosma arhinia microphthalmia syndrome (BAMS). Without maternal SMCHD1 in the egg cell, children bear with altered skeletal structures.

Background References

1. Gurzau AD et al. Relating SMCHD1 structure to its function in epigenetic silencing. Biochem Soc Trans. 2020 Aug

2. Gurzau AD et al. SMCHD1\'s ubiquitin-like domain is required for N-terminal dimerization and chromatin localization. Biochem J. 2021 Jul

Subcellular Location

Chromosome.

UNIPROT

SwissProt: A6NHR9 Human

Synonyms

BAMS antibody

FSHD2 antibody

KIAA0650 antibody

SMC hinge domain containing 1 antibody

SMC hinge domain containing protein 1 antibody

Smchd1 antibody

SMHD1_HUMAN antibody

Structural maintenance of chromosomes flexible hinge domain containing 1 antibody

Structural maintenance of chromosomes flexible hinge domain-containing protein 1 antibody

Images

  • Western blot analysis of SMCHD1 on different lysates with Rabbit anti-SMCHD1 antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: U-87 MG cell lysate<br />Lane 5: U-2 OS cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 226 kDa<br />Observed band size: 226 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SMCHD1 on different lysates with Rabbit anti-SMCHD1 antibody (HA722141) at 1/1,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: U-87 MG cell lysate
    Lane 5: U-2 OS cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 226 kDa
    Observed band size: 226 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722141) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SMCHD1 antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SMCHD1 antibody (HA722141) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722141) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SMCHD1 antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SMCHD1 antibody (HA722141) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722141) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SMCHD1 antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722141" style="font-weight: bold;text-decoration: underline;">HA722141</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SMCHD1 antibody (HA722141) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722141) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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