GATA2 Recombinant Rabbit Monoclonal Antibody [PSH04-53]
Catalog# HA722152
GATA2 Recombinant Rabbit Monoclonal Antibody [PSH04-53]
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WB
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IF-Cell
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Human
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Rat
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unconjugated
Overview
Product Name
GATA2 Recombinant Rabbit Monoclonal Antibody [PSH04-53]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human GATA2 aa 1-50.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
SH-SY5Y cell lysate, LNCaP cell lysate, PC-12 cell lysate, SH-SY5Y.
Conjugation
unconjugated
Clone Number
PSH04-53
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
Target
Function
GATA2 or GATA-binding factor 2 is a transcription factor, i.e. a nuclear protein which regulates the expression of genes. It regulates many genes that are critical for the embryonic development, self-renewal, maintenance, and functionality of blood-forming, lympathic system-forming, and other tissue-forming stem cells. GATA2 is encoded by the GATA2 gene, a gene which often suffers germline and somatic mutations which lead to a wide range of familial and sporadic diseases, respectively. The gene and its product are targets for the treatment of these diseases. Inactivating mutations of the GATA2 gene cause a reduction in the cellular levels of GATA2 and the development of a wide range of familial hematological, immunological, lymphatic, and/or other disorders that are grouped together into a common disease termed GATA2 deficiency. Less commonly, these disorders are associated with non-familial (i.e. sporadic or acquired) GATA inactivating mutations. GATA2 deficiency often begins with seemingly benign abnormalities but if untreated progresses to life-threatening opportunistic infections, virus-induced cancers, lung failure, the myelodysplastic syndrome (i.e. MDS), and/or acute myeloid leukemia, principally acute myeloid leukemia (AML), less commonly chronic myelomonocytic leukemia (CMML), and rarely a lymphoid leukemia.
Background References
1. Calvo KR et al. The spectrum of GATA2 deficiency syndrome. Blood. 2023 Mar
2. Fabozzi F et al. GATA2 and marrow failure. Best Pract Res Clin Haematol. 2021 Jun
Subcellular Location
Nucleus.
Synonyms
DCML antibody
Endothelial transcription factor GATA 2 antibody
Endothelial transcription factor GATA-2 antibody
Endothelial transcription factor GATA2 antibody
FLJ45948 antibody
GATA 2 antibody
GATA binding protein 2 antibody
GATA-binding protein 2 antibody
Gata2 antibody
GATA2_HUMAN antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of GATA2 on different lysates with Rabbit anti-GATA2 antibody (HA722152) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: LNCaP cell lysate
Lane 3: Jurkat cell lysate (negative)
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: Lane 1: 9 seconds; Lane 2-4: 40 seconds;
ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722152) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of SH-SY5Y (positive) and Jurkat (negative) labeling GATA2 with Rabbit anti-GATA2 antibody (HA722152) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GATA2 antibody (HA722152) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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