Nkx2.2 Recombinant Rabbit Monoclonal Antibody [JE60-66]
Catalog# HA722185
Nkx2.2 Recombinant Rabbit Monoclonal Antibody [JE60-66]
-
WB
-
IF-Cell
-
IHC-P
-
IF-Tissue
-
IHC-Fr
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Nkx2.2 Recombinant Rabbit Monoclonal Antibody [JE60-66]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Nkx2.2 aa 1-273.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, IF-Tissue, IHC-Fr
Molecular Weight
Predicted band size: 30 kDa
Positive Control
U-2 OS cell lysate, C6 cell lysate, rat brain tissue lysate, C6, human pancreas tissue, mouse pancreas tissue, rat brian tissue.
Conjugation
unconjugated
Clone Number
JE60-66
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IF-Cell
-
1:100
-
IHC-P
-
1:500-1:1,000
-
IF-Tissue
-
1:200-1:500
-
IHC-Fr
-
1:500
Target
Function
Homeobox protein Nkx-2.2 is a protein that in humans is encoded by the NKX2-2 gene. Homeobox protein Nkx-2.2 contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. The expression of Nkx2-2 is regulated by an antisense RNA called Nkx2-2as. In the developing spinal cord, Nkx-2.2 regulates IRX3 thereby contributing to the proper differentiation of the ventral horn neurons.
Background References
1. Mariyath MPM et al. Novel Homeodomain Transcription Factor Nkx2.2 in the Brain Tumor Development. Curr Cancer Drug Targets. 2020
2. Seymour PA et al. Differential use of the Nkx2.2 NK2 domain in developing pancreatic islets and neurons. Genes Dev. 2023 Jun
Subcellular Location
Nucleus.
Synonyms
Homeobox protein NK 2 homolog B antibody
Homeobox protein NK-2 homolog B antibody
Homeobox protein Nkx 2.2 antibody
Homeobox protein Nkx-2.2 antibody
NK 2 homolog B antibody
NK2 homeobox 2 antibody
NK2 transcription factor like protein B antibody
NK2 transcription factor related locus 2 antibody
NKX2 2 antibody
NKX2-2 antibody
ExpandImages
-
Application: IHC-Fr
Species: Mouse
Site: E14.5 embyro
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IF-Tissue
Species: Mouse
Site: pancreas
Sample: Paraffin-embedded section
Antibody concentration: 1/200 -
Western blot analysis of Nkx2.2 on different lysates with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/1,000 dilution.
Lane 1: U-2 OS cell lysate (20 µg/Lane)
Lane 2: C6 cell lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 30 kDa
Observed band size: 34 kDa
Exposure time: 1 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722185) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of C6 cells labeling Nkx2.2 with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722185) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722185) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722185) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse P0 brain tissue with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722185) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse P0 brain tissue with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722185) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722185) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722185) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"