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Human MBL2 Recombinant Rabbit Monoclonal Antibody [PSH04-86] - BSA and Azide free (Detector)

Usd: 649

Specification

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Catalog# HA722192

Human MBL2 Recombinant Rabbit Monoclonal Antibody [PSH04-86] - BSA and Azide free (Detector)

Application

  • ELISA(Det)

Reactivity

  • Human

Pair: Capture
Pair: Detector
Pair: Protein
Range

  • 99-8,000 pg/mL

PI

  • 6.08

Conjugation

  • unconjugated

Overview

Product Name

Human MBL2 Recombinant Rabbit Monoclonal Antibody [PSH04-86] - BSA and Azide free (Detector)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MBL2 aa 108-248 (P11226).

Species Reactivity

Human

Validated Applications

ELISA(Det)

Molecular Weight

Predicted band size: 26 kDa

Positive Control

Recombinant Human MBL2 protein (HA210628).

Conjugation

unconjugated

Clone Number

PSH04-86

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Det)

  • Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-85] to Human MBL2 (Capture) (HA722191) and recombinant standard Human MBL2 protein (HA210628). The reference range value is 99-8,000 pg/mL.

Target

Function

Mannose-binding lectin (MBL), also called mannan-binding lectin or mannan-binding protein (MBP), is a lectin that is instrumental in innate immunity as an opsonin and via the lectin pathway. MBL belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL recognizes carbohydrate patterns found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding of MBL to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Background References

1. Yuan ZC et al. Association of MBL2 gene polymorphisms and systemic lupus erythematosus susceptibility: A meta-analysis. Int J Rheum Dis. 2021 Feb

2. Sokkar MF et al. MBL2 gene variants and susceptibility to meningitis in Egyptian patients. Gene. 2023 Jul

Subcellular Location

Secreted.

UNIPROT

SwissProt: P11226 Human

Synonyms

COLEC 1 antibody

COLEC1 antibody

Collectin-1 antibody

HSMBPC antibody

Lectin, mannose-binding, soluble, 2 antibody

Mannan binding lectin antibody

Mannan binding protein antibody

Mannan-binding protein antibody

Mannose binding lectin (protein C) 2 soluble antibody

Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody

Expand

Images

  • Sandwich ELISA analysis of Human MBL2 matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722191" style="font-weight: bold;text-decoration: underline;">HA722191</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MBL2 protein (<a href="/products/HA210628" style="font-weight: bold;text-decoration: underline;">HA210628</a>) starting from 8000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722192" style="font-weight: bold;text-decoration: underline;">HA722192</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of Human MBL2 matched pair antibodies

    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722191) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MBL2 protein (HA210628) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722192, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • The concentrations of MBL2 were interpolated from the MBL2 standard curves and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 100%. The mean MBL2 concentration was determined to be 1,936 pg/ml in HepG2 cell culture supernatant.

    The concentrations of MBL2 were interpolated from the MBL2 standard curves and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 100%. The mean MBL2 concentration was determined to be 1,936 pg/ml in HepG2 cell culture supernatant.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"