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TFPI Recombinant Rabbit Monoclonal Antibody [JE40-58]

Usd: 385

Specification

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Catalog# HA722199

TFPI Recombinant Rabbit Monoclonal Antibody [JE40-58]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TFPI Recombinant Rabbit Monoclonal Antibody [JE40-58]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human TFPI aa 250-304.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 35 kDa

Positive Control

HepG2 cell lysate, HUVEC cell lysate, EA.hy926 cell lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

JE40-58

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • IP

  • 1-2μg/sample

Target

Function

Tissue factor pathway inhibitor (or TFPI) is a single-chain polypeptide which can reversibly inhibit factor Xa (Xa). While Xa is inhibited, the Xa-TFPI complex can subsequently also inhibit the FVIIa-tissue factor complex. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM. TFPI has a relative molecular mass of 34,000 to 40,000 depending on the degree of proteolysis of the C-terminal region. TFPI consists of a highly negatively charged amino-terminus, three tandemly linked Kunitz domains, and a highly positively charged carboxy-terminus. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypsin inhibitor and bovine basic pancreatic trypsin inhibitor.

Background References

1. Luo J et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022 Mar

2. Xing M et al. TFPI inhibits breast cancer progression by suppressing ERK/p38 MAPK signaling pathway. Genes Genomics. 2022 Jul

Subcellular Location

Secreted, Microsome membrane.

UNIPROT

SwissProt: P10646 Human

Synonyms

Anti convertin antibody

EPI antibody

Extrinsic pathway inhibitor antibody

LACI antibody

Lipoprotein associated coagulation inhibitor antibody

Lipoprotein-associated coagulation inhibitor antibody

TFI antibody

TFPI 1 antibody

TFPI antibody

TFPI1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TFPI on different lysates with Rabbit anti-TFPI antibody (<a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: LoVo cell lysate (negative)<br />Lane 3: HUVEC cell lysate<br />Lane 4: EA.hy926 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35-45 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TFPI on different lysates with Rabbit anti-TFPI antibody (HA722199) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: LoVo cell lysate (negative)
    Lane 3: HUVEC cell lysate
    Lane 4: EA.hy926 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35-45 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722199) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • TFPI was immunoprecipitated from 0.2 mg HepG2 cell lysate with <a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HepG2 cell lysate (input)<br />Lane 2: <a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a> IP in HepG2 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a> in HepG2 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 52 seconds; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    TFPI was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA722199 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722199 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HepG2 cell lysate (input)
    Lane 2: HA722199 IP in HepG2 cell lysate
    Lane 3: Rabbit IgG instead of HA722199 in HepG2 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 52 seconds; ECL: K1802

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI antibody (<a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722199" style="font-weight: bold;text-decoration: underline;">HA722199</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI antibody (HA722199) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722199) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"