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ZBTB7A Recombinant Rabbit Monoclonal Antibody [JE34-74]

Usd: 385

Specification

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Catalog# HA722205

ZBTB7A Recombinant Rabbit Monoclonal Antibody [JE34-74]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ZBTB7A Recombinant Rabbit Monoclonal Antibody [JE34-74]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, IP

Molecular Weight

Predicted band size: 61 kDa

Positive Control

MCF7 cell lysate, HepG2 cell lysate, K-562 cell lysate, HeLa cell lysate, HeLa, human tonsil tissue.

Conjugation

unconjugated

Clone Number

JE34-74

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Zinc finger and BTB domain-containing protein 7A is a protein that in humans is encoded by the ZBTB7A gene. Enables several functions, including SMAD binding activity; androgen receptor binding activity; and transcription corepressor binding activity. Involved in several processes, including erythrocyte maturation; negative regulation of signal transduction; and regulation of nucleobase-containing compound metabolic process. Located in cytoplasm and nucleus. Colocalizes with DNA-dependent protein kinase complex and NuRD complex.

Background References

1. Singh AK et al. Role of ZBTB7A zinc finger in tumorigenesis and metastasis. Mol Biol Rep. 2021 May

2. Jeong JH et al. ZBTB7A suppresses glioblastoma tumorigenesis through the transcriptional repression of EPB41L5. Exp Mol Med. 2023 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O95365 Human

Synonyms

DKFZp547O146 antibody

Factor binding IST protein 1 antibody

Factor that binds to inducer of short transcripts protein 1 antibody

FBI-1 antibody

FBI1 antibody

HIV-1 1st-binding protein 1 antibody

HIV-1 inducer of short transcripts binding protein antibody

HIV-1 inducer of short transcripts-binding factor 1 antibody

Leukemia/lymphoma-related factor antibody

LRF antibody

Expand

Images

  • Western blot analysis of ZBTB7A on different lysates with Rabbit anti-ZBTB7A antibody (<a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 61 kDa<br />Observed band size: 61 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ZBTB7A on different lysates with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

    Exposure time: 15 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722205) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling ZBTB7A with Rabbit anti-ZBTB7A antibody (<a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a>) at 1/1,000 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZBTB7A antibody (<a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a>) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling ZBTB7A with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ZBTB7A antibody (<a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722205) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ZBTB7A was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722205" style="font-weight: bold;text-decoration: underline;">HA722205</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    ZBTB7A was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722205 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722205 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA722205 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA722205 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute; ECL: K1802

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