VAV3 Recombinant Rabbit Monoclonal Antibody [JE33-02]
Catalog# HA722212
VAV3 Recombinant Rabbit Monoclonal Antibody [JE33-02]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
VAV3 Recombinant Rabbit Monoclonal Antibody [JE33-02]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human VAV3 aa 748-847 / 847.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 98 kDa
Positive Control
Jurkat cell lysate, HeLa cell lysate, MCF7 cell lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE33-02
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Guanine nucleotide exchange factor VAV3 is a protein that in humans is encoded by the VAV3 gene. This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. VAV3 has been shown to interact with Grb2.
Background References
1. Al-Hawary SIS et al. VAV3 in human cancers: Mechanism and clinical implication. Pathol Res Pract. 2023 Aug
2. Gleeson PJ et al. CARD9, VAV3, and infection risk in IgA nephropathy. Kidney Int. 2023 May
Subcellular Location
Cytoplasm, cytosol, immunological synapse, plasma membrane.
Synonyms
FLJ40431 antibody
Guanine nucleotide exchange factor VAV3 antibody
Protein vav 3 antibody
Protein vav3 antibody
RGD1565941 antibody
VAV 3 antibody
Vav 3 guanine nucleotide exchange factor antibody
VAV 3 oncogene antibody
VAV 3 protein antibody
VAV-3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of VAV3 on different lysates with Rabbit anti-VAV3 antibody (HA722212) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (low expression) (20 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 98 kDa
Observed band size: 98 kDa
Exposure time: 46 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722212) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"