TFIIB Recombinant Rabbit Monoclonal Antibody [JE38-37]
Catalog# HA722213
TFIIB Recombinant Rabbit Monoclonal Antibody [JE38-37]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TFIIB Recombinant Rabbit Monoclonal Antibody [JE38-37]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TFIIB aa 217-316 / 316.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, C6 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE38-37
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Transcription factor II B (TFIIB) is a general transcription factor that is involved in the formation of the RNA polymerase II preinitiation complex (PIC) and aids in stimulating transcription initiation. TFIIB is localised to the nucleus and provides a platform for PIC formation by binding and stabilising the DNA-TBP (TATA-binding protein) complex and by recruiting RNA polymerase II and other transcription factors. It is encoded by the TFIIB gene, and is homologous to archaeal transcription factor B and analogous to bacterial sigma factors.
Background References
1. O\'Brien MJ et al. Beyond the canonical role of TFIIB in eukaryotic transcription. Curr Genet. 2022 Feb
2. O\'Brien MJ et al. Critical Involvement of TFIIB in Viral Pathogenesis. Front Mol Biosci. 2021 Apr
Subcellular Location
Nucleus, Chromosome.
Synonyms
General transcription factor IIB antibody
General transcription factor TFIIB antibody
gtf2b antibody
RNA polymerase II transcription factor IIB antibody
S300 II antibody
S300-II antibody
TF IIB antibody
TF2B antibody
TF2B_HUMAN antibody
TFIIB antibody
ExpandImages
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Western blot analysis of TFIIB on different lysates with Rabbit anti-TFIIB antibody (HA722213) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: K-562 cell lysate
Lane 5: C6 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722213) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"