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Cleaved PARP1 Recombinant Rabbit Monoclonal Antibody [JE00-15]

Usd: 385

Specification

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Catalog# HA722218

Cleaved PARP1 Recombinant Rabbit Monoclonal Antibody [JE00-15]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cleaved PARP1 Recombinant Rabbit Monoclonal Antibody [JE00-15]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PARP1 aa 164-213.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB

Molecular Weight

Predicted band size: 27 kDa

Positive Control

Jurkat cell lysate, Jurkat treated with 1μM staurosporine for 3 hours cell lysate, HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate.

Conjugation

unconjugated

Clone Number

JE00-15

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, genomic stability, and programmed cell death. The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.

Background References

1. Peh J et al. The Combination of Vemurafenib and Procaspase-3 Activation Is Synergistic in Mutant BRAF Melanomas. Mol Cancer Ther 15:1859-69 (2016).

2. Gao L et al. Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats. Front Pharmacol 6:215 (2015).

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P09874 Human

SwissProt: P11103 Mouse

SwissProt: P27008 Rat

Synonyms

ADP ribosyltransferase diphtheria toxin like 1 antibody

ADP ribosyltransferase NAD(+) antibody

ADP-ribosyltransferase diphtheria toxin-like 1 antibody

ADPRT 1 antibody

ADPRT antibody

ADPRT1 antibody

APOPAIN antibody

ARTD1 antibody

NAD(+) ADP-ribosyltransferase 1 antibody

PARP antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Cleaved PARP1 on different lysates with Rabbit anti-Cleaved PARP1 antibody (<a href="/products/HA722218" style="font-weight: bold;text-decoration: underline;">HA722218</a>) at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: Jurkat treated with 1μM staurosporine for 3 hours cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: Neuro-2a cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722218" style="font-weight: bold;text-decoration: underline;">HA722218</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Cleaved PARP1 on different lysates with Rabbit anti-Cleaved PARP1 antibody (HA722218) at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: Jurkat treated with 1μM staurosporine for 3 hours cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: Neuro-2a cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722218) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation