CCT2 Recombinant Rabbit Monoclonal Antibody [JE37-96]
Catalog# HA722240
CCT2 Recombinant Rabbit Monoclonal Antibody [JE37-96]
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WB
-
IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CCT2 Recombinant Rabbit Monoclonal Antibody [JE37-96]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CCT2 aa 500-535.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE37-96
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IP
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1-2μg/sample
Target
Function
T-complex protein 1 subunit beta is a protein that in humans is encoded by the CCT2 gene. This gene encodes a molecular chaperone that is member of the TRiC complex. This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized. CCT2 (gene) has been shown to interact with PPP4C.
Background References
1. Ma X et al. CCT2 is an aggrephagy receptor for clearance of solid protein aggregates. Cell. 2022 Apr
2. Zhang Z et al. CCT2, a newly identified aggrephagy receptor in mammals, specifically mediates the autophagic clearance of solid protein aggregates. Autophagy. 2022 Jul
Subcellular Location
Cytoplasm.
Synonyms
99D8.1 antibody
CCT 2 antibody
CCT beta antibody
CCT-beta antibody
CCT2 antibody
CCTB antibody
Chaperonin containing t complex polypeptide 1 beta subunit antibody
Chaperonin containing t complex polypeptide 1 subunit 2 antibody
Chaperonin containing TCP1 subunit 2 antibody
Chaperonin containing TCP1 subunit 2 (beta) antibody
ExpandImages
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Western blot analysis of CCT2 on different lysates with Rabbit anti-CCT2 antibody (HA722240) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 50 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722240) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
CCT2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722240 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722240 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA722240 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722240 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 5 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"