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Phospho-TAK1 (S439) Recombinant Rabbit Monoclonal Antibody [JE44-93]

Usd: 385

Specification

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Catalog# HA722242

Phospho-TAK1 (S439) Recombinant Rabbit Monoclonal Antibody [JE44-93]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospho-TAK1 (S439) Recombinant Rabbit Monoclonal Antibody [JE44-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser439 of mouse TAK1.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 67 kDa

Positive Control

HeLa cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, K-562 cell lysate, C2C12 cell lysate, C6 cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate.

Conjugation

unconjugated

Clone Number

JE44-93

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:25,000

Target

Function

Mitogen-activated protein kinase kinase kinase 7 (MAP3K7), also known as TAK1, is an enzyme that in humans is encoded by the MAP3K7 gene. This kinase has also been shown to regulate downstream cytokine expression such as TNF. Due to its regulation of TNF, TAK1 has become a novel target for the treatment of TNF mediated diseases such as auto immune disease ( Rheumatoid Arthritis, lupus, IBD) but also other cytokine mediated disorders such as chronic pain and cancer. With the advent of novel selective TAK1 inhibitors, groups have explored the therapeutic potential of TAK1 targeted therapies. One group has shown that the selective TAK1 inhibitor, Takinib developed at Duke University attenuated rheumatoid arthritis like pathology in the CIA mouse model of human inflammatory arthritis. Furthermore, pharmacological inhibition of TAK1 has shown to reduce inflammatory cytokines in particular TNF.

Background References

1. Ma M et al. TAK1 is an essential kinase for STING trafficking. Mol Cell. 2023 Nov

2. Su W et al. TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling. JHEP Rep. 2023 Feb

Subcellular Location

Cytoplasm, Cell membrane.

UNIPROT

SwissProt: O43318 Human

SwissProt: Q62073 Mouse

SwissProt: P0C8E4 Rat

Synonyms

M3K7_HUMAN antibody

MAP3K 7 antibody

Map3k7 antibody

MEKK7 antibody

Mitogen activated protein kinase kinase kinase 7 antibody

Mitogen-activated protein kinase kinase kinase 7 antibody

TAK1 antibody

TGF beta activated kinase 1 antibody

TGF-beta-activated kinase 1 antibody

TGF1a antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (<a href="/products/HA722242" style="font-weight: bold;text-decoration: underline;">HA722242</a>) at 1/25,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: C2C12 cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate<br />Lane 8: PC-12 cell lysate<br />Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 67 kDa<br />Observed band size: 67 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722242" style="font-weight: bold;text-decoration: underline;">HA722242</a>) at 1/25,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/25,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: C2C12 cell lysate
    Lane 3: C6 cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate
    Lane 8: PC-12 cell lysate
    Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (<a href="/products/HA722242" style="font-weight: bold;text-decoration: underline;">HA722242</a>) at 1/5,000 dilution and pan TAK1 antibody (<a href="/products/ET1705-14" style="font-weight: bold;text-decoration: underline;">ET1705-14</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate<br />Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 67 kDa<br />Observed band size: 67 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722242" style="font-weight: bold;text-decoration: underline;">HA722242</a>) at 1/5,000 dilution and pan TAK1 antibody (<a href="/products/ET1705-14" style="font-weight: bold;text-decoration: underline;">ET1705-14</a>) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate
    Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"