Phospho-GCN2 (T899) Recombinant Rabbit Monoclonal Antibody [JE00-51]
Catalog# HA722249
Phospho-GCN2 (T899) Recombinant Rabbit Monoclonal Antibody [JE00-51]
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WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Phospho-GCN2 (T899) Recombinant Rabbit Monoclonal Antibody [JE00-51]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr899 of Human GCN2.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 187 kDa
Positive Control
HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, Neuro-2a treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate.
Conjugation
unconjugated
Clone Number
JE00-51
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000-1:2,000
Target
Function
The family of stress-responsive protein kinases include HRI (heme-regulated inhibitor or EIF2AK1), PKR (EIF2AK2 or TIK), PERK (EIF2AK3) and GCN2 (EIF2AK4). These proteins phosphorylate the eukaryotic translation initiation factor 2α (eIF2α) on Ser 51 to regulate general and gene-specific protein synthesis. Phosphorylated eIF2α acts as an inhibitor of its guanine nucleotide exchange factor eIF2B. GCN2, a unique eIF2α kinase, exists in all eukaryotes from yeast to mammals. In mammals, expression of GCN2 is highest in liver and brain tissues. GCN2 primarily initiates the phosphorylation of eIF2α in response to UV, but has been shown to increase phosphorylation activity in response to serum starvation. Also, substitution of Asp 83 for Ala on eIF2α results in impaired phosphorylation by GCN2 and PKR, suggesting a contribution of remote residues to kinase-substrate recognition.
Background References
1. Li C et al. Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2alpha axis. Cell Stem Cell. 2022 Jul
2. Missiaen R et al. GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment. Cell Metab. 2022 Aug
Subcellular Location
Cytoplasm.
Synonyms
E2AK4_HUMAN antibody
Eif2ak4 antibody
Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
GCN2 antibody
GCN2 eIF2alpha kinase antibody
GCN2 like protein antibody
GCN2-like protein antibody
KIAA1338 antibody
MGCN2 antibody
Images
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☑ Cell treatment (CT)
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody (HA722249) at 1/1,000 dilution and pan GCN2 antibody (ET1704-68) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (25 µg/Lane)
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate (25 µg/Lane)
Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour (25 µg/Lane)
Predicted band size: 187 kDa
Observed band size: 187 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722249) at 1/1,000 dilution and pan GCN2 antibody (ET1704-68) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody (HA722249) at 1/2,000 dilution.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: Neuro-2a treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane)
Lane 1: C6 cell lysate (20 µg/Lane)
Lane 2: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate (20 µg/Lane)
Predicted band size: 187 kDa
Observed band size: 187 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722249) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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