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Munc18-1 Recombinant Rabbit Monoclonal Antibody [JE38-54]

Usd: 385

Specification

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Catalog# HA722250

Munc18-1 Recombinant Rabbit Monoclonal Antibody [JE38-54]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Munc18-1 Recombinant Rabbit Monoclonal Antibody [JE38-54]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Munc18-1 aa 401-500 / 594.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 68 kDa

Positive Control

SH-SY5Y cell lysate, SiHa cell lysate, HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human testis tissue, human brain tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

JE38-54

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:200-1:1,000

  • IF-Tissue

  • 1:50-1:200

Target

Function

Syntaxin-binding protein 1 (also known as Munc18-1) is a protein that in humans is encoded by the STXBP1 gene. This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with neurological disorders including epilepsy, intellectual disability, and movement disorders. The encoded protein may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. It can interact with syntaxins 1, 2, and 3 but not syntaxin 4 and may play a role in determining the specificity of intracellular fusion reactions. This protein functions in a late stage of the intracellular membrane fusion process of exocytosis. Dissociation of this protein from syntaxin determines the kinetics of postfusion events. This protein is essential for presynpatic vesicle release and is rapidly phosphorylated by protein kinase C upon neuronal depolarization. The protein participates in the secretory pathway between the Golgi apparatus and cell membrane.

Background References

1. Wang S et al. Neuronal SNARE complex assembly guided by Munc18-1 and Munc13-1. FEBS Open Bio. 2022 Nov

2. Puntman DC et al. Munc18-1 is essential for neuropeptide secretion in neurons. J Neurosci. 2021 Jun

Subcellular Location

Cytoplasm, cytosol, Membrane.

UNIPROT

SwissProt: P61764 Human

SwissProt: O08599 Mouse

SwissProt: P61765 Rat

Synonyms

FLJ37475 antibody

Munc 18 1 antibody

Munc 18a antibody

MUNC18 1 antibody

N-Sec1 antibody

Neuronal SEC1 antibody

NSec1 antibody

p67 antibody

Protein unc-18 homolog 1 antibody

Protein unc-18 homolog A antibody

Expand

Images

  • Western blot analysis of Munc18-1 on different lysates with Rabbit anti-Munc18-1 antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: SiHa cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: K-562 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 68 kDa<br />Observed band size: 68 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Munc18-1 on different lysates with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: SiHa cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: K-562 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722250) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Munc18-1 on different lysates with Rabbit anti-Munc18-1 antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/5,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: MEF cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: Mouse brain tissue lysate<br />Lane 5: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 68 kDa<br />Observed band size: 68 kDa<br /><br />Exposure time: Lane 1-3: 20 seconds; Lane 4-5: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Munc18-1 on different lysates with Rabbit anti-Munc18-1 antibody (HA722250) at 1/5,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: MEF cell lysate
    Lane 3: C6 cell lysate
    Lane 4: Mouse brain tissue lysate
    Lane 5: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

    Exposure time: Lane 1-3: 20 seconds; Lane 4-5: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722250) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Munc18-1 antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Munc18-1 antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Munc18-1 antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Munc18-1 antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722250" style="font-weight: bold;text-decoration: underline;">HA722250</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation