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DNA Polymerase lambda Recombinant Rabbit Monoclonal Antibody [PSH05-24]

Usd: 385

Specification

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Catalog# HA722253

DNA Polymerase lambda Recombinant Rabbit Monoclonal Antibody [PSH05-24]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

DNA Polymerase lambda Recombinant Rabbit Monoclonal Antibody [PSH05-24]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human DNA Polymerase lambda aa 1-575 / 575.

Species Reactivity

Human, Mouse, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 63 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, 293T cell lysate, HepG2 cell lysate, mouse testis tissue lysate, COS-1 cell lysate, human testis tissue.

Conjugation

unconjugated

Clone Number

PSH05-24

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

Target

Function

DNA polymerase lambda, also known as Pol λ, is an enzyme found in all eukaryotes. In humans, it is encoded by the POLL gene. Pol λ is a member of the X family of DNA polymerases. It is thought to resynthesize missing nucleotides during non-homologous end joining (NHEJ), a pathway of DNA double-strand break (DSB) repair. NHEJ is the main pathway in higher eukaryotes for repair of DNA DSBs. Chromosomal DSBs are the most severe type of DNA damage. During NHEJ, duplexes generated by the alignment of broken DNA ends usually contain small gaps that need to be filled in by a DNA polymerase. DNA polymerase lambda can perform this function.

Background References

1. Maltseva EA et al. Non-Catalytic Domains of DNA Polymerase lambda: Influence on Enzyme Activity and Its Regulation. Dokl Biochem Biophys. 2023 Oct

2. Kaminski AM et al. DNA polymerase lambda Loop1 variant yields unexpected gain-of-function capabilities in nonhomologous end-joining. DNA Repair (Amst). 2024 Feb

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: Q9UGP5 Human

SwissProt: Q9QXE2 Mouse

Synonyms

BETA N antibody

BETAN antibody

DNA directed DNA polymerase lambda antibody

DNA polymerase beta 2 antibody

DNA polymerase beta-2 antibody

DNA polymerase kappa antibody

DNA polymerase kappa DNA polymerase beta N antibody

DNA polymerase lambda antibody

DNA polymerase lamda2 antibody

DPOLL_HUMAN antibody

Expand

Images

  • Western blot analysis of DNA Polymerase lambda on different lysates with Rabbit anti-DNA Polymerase lambda antibody (<a href="/products/HA722253" style="font-weight: bold;text-decoration: underline;">HA722253</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane)<br />Lane 4: HepG2 cell lysate (20 µg/Lane)<br />Lane 5: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 6: COS-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 63 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 2 minutes 22 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722253" style="font-weight: bold;text-decoration: underline;">HA722253</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DNA Polymerase lambda on different lysates with Rabbit anti-DNA Polymerase lambda antibody (HA722253) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: HepG2 cell lysate (20 µg/Lane)
    Lane 5: Mouse testis tissue lysate (40 µg/Lane)
    Lane 6: COS-1 cell lysate (20 µg/Lane)

    Predicted band size: 63 kDa
    Observed band size: 70 kDa

    Exposure time: 2 minutes 22 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722253) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DNA Polymerase lambda antibody (<a href="/products/HA722253" style="font-weight: bold;text-decoration: underline;">HA722253</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722253" style="font-weight: bold;text-decoration: underline;">HA722253</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DNA Polymerase lambda antibody (HA722253) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722253) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"