TRIF Recombinant Rabbit Monoclonal Antibody [PSH05-39]
Catalog# HA722268
TRIF Recombinant Rabbit Monoclonal Antibody [PSH05-39]
-
WB
-
Human
-
HA750998
不含抗保成分
-
unconjugated
Overview
Product Name
TRIF Recombinant Rabbit Monoclonal Antibody [PSH05-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TRIF aa 351-650 / 712.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 76 kDa
Positive Control
Raji cell lysate, Jurkat cell lysate, THP-1 cell lysate, HeLa cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Clone Number
PSH05-39
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
TIR domain containing adaptor molecule 1 (TICAM1; formerly known as TIR-domain-containing adapter-inducing interferon-β or TRIF) is an adapter in responding to activation of toll-like receptors (TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. Toll-like receptors (TLRs) recognize specific components of microbial invaders and activate an immune response to these pathogens. After these receptors recognize highly conserved pathogenic patterns, a downstream signaling cascade is activated in order to stimulate the release of inflammatory cytokines and chemokines as well as to upregulate the expression of immune cells. All TLRs have a TIR domain that initiates the signaling cascade through TIR adapters. Adapters are platforms that organize downstream signaling cascades leading to a specific cellular response after exposure to a given pathogen.
Background References
1. Muendlein HI et al. Neutrophils and macrophages drive TNF-induced lethality via TRIF/CD14-mediated responses. Sci Immunol. 2022 Dec
2. Pereira M et al. The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways. Cell Rep. 2022 Aug
Subcellular Location
Cytoplasmic vesicle, autophagosome, Cytoplasm, cytosol, Mitochondrion.
Synonyms
IIAE6 antibody
MGC35334 antibody
MyD88 3 antibody
Proline rich vinculin and TIR domain containing protein B antibody
Proline-rich antibody
PRVTIRB antibody
Putative NF kappa B activating protein 502H antibody
Putative NF-kappa-B-activating protein 502H antibody
Putative NFkB activating protein antibody
TCAM1_HUMAN antibody
ExpandImages
-
Western blot analysis of TRIF on different lysates with Rabbit anti-TRIF antibody (HA722268) at 1/2,000 dilution.
Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: THP-1 cell lysate
Lane 4: HeLa cell lysate
Lane 5: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 100 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722268) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
TRIF Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
