Kaiso Recombinant Rabbit Monoclonal Antibody [JE38-42]
Catalog# HA722275
Kaiso Recombinant Rabbit Monoclonal Antibody [JE38-42]
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WB
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IF-Cell
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FC
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Human
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Rat
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unconjugated
Overview
Product Name
Kaiso Recombinant Rabbit Monoclonal Antibody [JE38-42]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Kaiso aa 600-700.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 74 kDa
Positive Control
MCF7 cell lysate, HeLa cell lysate, HCT 116 cell lysate, C6 cell lysate, HeLa, C6.
Conjugation
unconjugated
Clone Number
JE38-42
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
Transcriptional regulator Kaiso is a protein that in humans is encoded by the ZBTB33 gene. This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.
Background References
1. Kaplun D et al. Kaiso Regulates DNA Methylation Homeostasis. Int J Mol Sci. 2021 Jul
2. Tian W et al. Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1. Mol Oncol. 2022 Sep
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
Kaiso antibody
Kaiso protein antibody
Kaiso transcription factor antibody
KAISO_HUMAN antibody
Transcriptional regulator Kaiso antibody
ZBTB 33 antibody
Zbtb33 antibody
Zinc finger and BTB domain containing 33 antibody
Zinc finger and BTB domain-containing protein 33 antibody
Zinc finger transcription factor Kaiso antibody
ExpandImages
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Western blot analysis of Kaiso on different lysates with Rabbit anti-Kaiso antibody (HA722275) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HCT 116 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722275) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling Kaiso with Rabbit anti-Kaiso antibody (HA722275) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Kaiso antibody (HA722275) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of C6 cells labeling Kaiso.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722275, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"