HMGA1 Recombinant Rabbit Monoclonal Antibody [JE58-17]
Catalog# HA722280
HMGA1 Recombinant Rabbit Monoclonal Antibody [JE58-17]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
HMGA1 Recombinant Rabbit Monoclonal Antibody [JE58-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human HMGA1 aa 1-80 / 107.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 12 kDa
Positive Control
A431 cell lysate, HCT 116 cell lysate, HepG2 cell lysate, MDA-MB-231 cell lysate, HCT 116.
Conjugation
unconjugated
Clone Number
JE58-17
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:200
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FC
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1:1,000
Target
Function
High-mobility group protein HMG-I/HMG-Y is a protein that in humans is encoded by the HMGA1 gene. This gene encodes a non-histone chromatin protein involved in many cellular processes, including regulation of inducible gene transcription, DNA replication, heterochromatin organization, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. HMGA1 proteins are quite small (~10-12 kDa) and basic molecules, and consist of three AT-hooks with the RGRP (Arg-Gly-Arg-Pro) core motif, a novel cross-linking domain located between the second and third AT-hook, and a C-terminal acidic tail characteristic for the HMG family comprising HMGA, HMGB and HMGN proteins.
Background References
1. Wang Y. et. al. HMGA1 in cancer: Cancer classification by location. J Cell Mol Med. 2019 Apr
2. Resar L. et. al. Lessons from the Crypt: HMGA1-Amping up Wnt for Stem Cells and Tumor Progression. Cancer Res. 2018 Apr
Subcellular Location
Nucleus, Chromosome.
UNIPROT
Synonyms
High mobility group (nonhistone chromosomal) protein isoforms I and Y antibody
High mobility group AT hook 1 antibody
High mobility group AT-hook protein 1 antibody
High mobility group protein 1 antibody
High mobility group protein A1 antibody
High mobility group protein HMG-I/HMG-Y antibody
High mobility group protein Ia antibody
High mobility group protein R antibody
HMG R antibody
HMG-I(Y) antibody
ExpandImages
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Western blot analysis of HMGA1 on different lysates with Rabbit anti-HMGA1 antibody (HA722280) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 12 kDa
Observed band size: 17 kDa
Exposure time: 1 minute 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722280) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HCT 116 cells labeling HMGA1 with Rabbit anti-HMGA1 antibody (HA722280) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HMGA1 antibody (HA722280) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HCT 116 cells labeling HMGA1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722280, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"