Skip to content

Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA722288

Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IP

Reactivity

  • Mouse

  • Rat

  • Pig

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Tau aa 571-620 / 758.

Species Reactivity

Mouse, Rat, Pig

Validated Applications

WB, IHC-P, IF-Tissue, IP

Molecular Weight

Predicted band size: 79 kDa

Positive Control

Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, Pig brain tissue lysate, rat kidney tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

PSH05-50

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:5,000

  • IF-Tissue

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

The tau proteins (abbreviated from tubulin associated unit are a group of six highly soluble protein isoforms produced by alternative splicing from the gene MAPT (microtubule-associated protein tau). They have roles primarily in maintaining the stability of microtubules in axons and are abundant in the neurons of the central nervous system (CNS), where the cerebral cortex has the highest abundance. They are less common elsewhere but are also expressed at very low levels in CNS astrocytes and oligodendrocytes. Pathologies and dementias of the nervous system such as Alzheimer's disease and Parkinson's disease are associated with tau proteins that have become hyperphosphorylated insoluble aggregates called neurofibrillary tangles. The tau proteins were identified in 1975 as heat-stable proteins essential for microtubule assembly, and since then they have been characterized as intrinsically disordered proteins.

Background References

1. Karikari TK et al. Blood phospho-tau in Alzheimer disease: analysis, interpretation, and clinical utility. Nat Rev Neurol. 2022 Jul

2. Palmqvist S et al. Prediction of future Alzheimer\'s disease dementia using plasma phospho-tau combined with other accessible measures. Nat Med. 2021 Jun

Subcellular Location

Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.

UNIPROT

SwissProt: P10637 Mouse

SwissProt: P19332 Rat

Synonyms

AI413597 antibody

AW045860 antibody

DDPAC antibody

FLJ31424 antibody

FTDP 17 antibody

G protein beta1/gamma2 subunit interacting factor 1 antibody

MAPT antibody

MAPTL antibody

MGC134287 antibody

MGC138549 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 2: Mouse spleen tissue lysate (negative) (20 µg/Lane)<br />Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane)<br />Lane 4: Mouse testis tissue lysate (negative) (20 µg/Lane)<br />Lane 5: Rat brain tissue lysate (20 µg/Lane)<br />Lane 6: Rat spleen tissue lysate (negative) (20 µg/Lane)<br />Lane 7: Rat cerebellum tissue lysate (20 µg/Lane)<br />Lane 8: Rat testis tissue lysate (negative) (20 µg/Lane)<br />Lane 9: Pig brain tissue lysate (20 µg/Lane)<br />Lane 10: Pig spleen tissue lysate (negative) (20 µg/Lane)<br /><br />Observed band size: 50-60 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.<br /><br />Negative control : Spleen, Testis (PMID: 24309898, PMID: 24386422)

    ☑ Relative expression (RE)

    Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (20 µg/Lane)
    Lane 2: Mouse spleen tissue lysate (negative) (20 µg/Lane)
    Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane)
    Lane 4: Mouse testis tissue lysate (negative) (20 µg/Lane)
    Lane 5: Rat brain tissue lysate (20 µg/Lane)
    Lane 6: Rat spleen tissue lysate (negative) (20 µg/Lane)
    Lane 7: Rat cerebellum tissue lysate (20 µg/Lane)
    Lane 8: Rat testis tissue lysate (negative) (20 µg/Lane)
    Lane 9: Pig brain tissue lysate (20 µg/Lane)
    Lane 10: Pig spleen tissue lysate (negative) (20 µg/Lane)

    Observed band size: 50-60 kDa

    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

    Negative control : Spleen, Testis (PMID: 24309898, PMID: 24386422)

  • Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (hot lysis)<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 79 kDa<br />Observed band size: 50-70 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate (hot lysis)
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 79 kDa
    Observed band size: 50-70 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 2: C2C12 cell lysate (negative) (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 79 kDa<br />Observed band size: 50-70 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (40 µg/Lane)
    Lane 2: C2C12 cell lysate (negative) (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 79 kDa
    Observed band size: 50-70 kDa

    Exposure time: 14 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Tau antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Tau antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Tau was immunoprecipitated from 0.2 mg mouse brain tissue lysate with <a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Mouse brain tissue lysate (input)<br />Lane 2: <a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a> IP in mouse brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722288" style="font-weight: bold;text-decoration: underline;">HA722288</a> in mouse brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 50 seconds; ECL: K1802

    Tau was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722288 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722288 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Mouse brain tissue lysate (input)
    Lane 2: HA722288 IP in mouse brain tissue lysate
    Lane 3: Rabbit IgG instead of HA722288 in mouse brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 50 seconds; ECL: K1802

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"