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Inhibin alpha Recombinant Rabbit Monoclonal Antibody [PD01-29]

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Specification

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Catalog# HA722301

Inhibin alpha Recombinant Rabbit Monoclonal Antibody [PD01-29]

Application

  • IHC-P

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Inhibin alpha Recombinant Rabbit Monoclonal Antibody [PD01-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Inhibin alpha aa 233-366.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, WB

Molecular Weight

Predicted band size: 40 kDa

Positive Control

Mouse ovary tissue lysate, mouse testis tissue lysate, rat ovary tissue lysate, rat testis tissue lysate, human adrenal gland tissue, human ovary granular cell tumor tissue.

Conjugation

unconjugated

Clone Number

PD01-29

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:500-1:1,000

  • WB

  • 1:1,000

Target

Function

Inhibin, alpha, also known as INHA, is a protein which in humans is encoded by the INHA gene. The inhibin alpha subunit joins either the beta A or beta B subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. However, in prostate cancer, expression of the inhibin alpha-subunit gene was suppressed and was not detectable in poorly differentiated tumor cells. Furthermore, because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone.

Background References

1. Mete O et al. Significance of Alpha-inhibin Expression in Pheochromocytomas and Paragangliomas. Am J Surg Pathol. 2021 Sep

2. Huang L et al. Elevated expression of inhibin alpha gene in sterile allotriploid crucian carp. Gen Comp Endocrinol. 2021 Oct

Subcellular Location

Secreted.

UNIPROT

SwissProt: P05111 Human

SwissProt: Q04997 Mouse

SwissProt: P17490 Rat

Synonyms

A inhibin subunit antibody

A inhibin subunit precursor antibody

AW55578 antibody

IHA antibody

inhA antibody

INHA_HUMAN antibody

Inhibin alpha chain antibody

Inhibin alpha chain precursor antibody

Inhibin alpha subunit antibody

Images

  • Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody (<a href="/products/HA722301" style="font-weight: bold;text-decoration: underline;">HA722301</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse ovary tissue lysate<br />Lane 2: Mouse testis tissue lysate<br />Lane 3: Rat ovary tissue lysate<br />Lane 4: Rat testis tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40-45 kDa<br /><br />Exposure time: 16 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722301" style="font-weight: bold;text-decoration: underline;">HA722301</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody (HA722301) at 1/1,000 dilution.

    Lane 1: Mouse ovary tissue lysate
    Lane 2: Mouse testis tissue lysate
    Lane 3: Rat ovary tissue lysate
    Lane 4: Rat testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 40 kDa
    Observed band size: 40-45 kDa

    Exposure time: 16 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722301) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-Inhibin alpha antibody (<a href="/products/HA722301" style="font-weight: bold;text-decoration: underline;">HA722301</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722301" style="font-weight: bold;text-decoration: underline;">HA722301</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-Inhibin alpha antibody (HA722301) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722301) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human ovary granular cell tumor tissue with Rabbit anti-Inhibin alpha antibody (<a href="/products/HA722301" style="font-weight: bold;text-decoration: underline;">HA722301</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722301" style="font-weight: bold;text-decoration: underline;">HA722301</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovary granular cell tumor tissue with Rabbit anti-Inhibin alpha antibody (HA722301) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722301) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"