TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH05-56]
Catalog# HA722303
TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH05-56]
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WB
-
Human
-
unconjugated
Overview
Product Name
TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH05-56]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TNFRSF14 aa 1-223 / 283.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 30 kDa
Positive Control
SK-MEL-28 cell lysate.
Conjugation
unconjugated
Clone Number
PSH05-56
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily encoded by the TNFRSF14 gene. This protein was originally known as herpesvirus entry mediator A (HveA); HveB and HveC are structurally unrelated proteins of the immunoglobulin superfamily. It is also known as CD270 in the cluster of differentiation classification. Moreover, it is also referred to as ATAR (another TRAF-associated receptor). The protein encoded by this gene is a member of the TNF-receptor superfamily. The cytoplasmic region of this receptor was found to bind to several TNF receptor associated factor (TRAF) family members, which may mediate the signal transduction pathways that activate the immune response. In melanocytic cells TNFRSF14 gene expression may be regulated by MITF. TNFRSF14 has been shown to interact with TRAF2, TNFSF14 and TRAF5.
Background References
1. Kanai S et al. Increased expression of TNFRSF14 and LIGHT in biliary epithelial cells of patients with primary sclerosing cholangitis. Dig Liver Dis. 2024 Feb
2. de Groen RAL et al. Frequent mutated B2M, EZH2, IRF8, and TNFRSF14 in primary bone diffuse large B-cell lymphoma reflect a GCB phenotype. Blood Adv. 2021 Oct
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
HVEML antibody
ATAR antibody
CD270 antibody
CD40 like protein precursor antibody
Herpes virus entry mediator A antibody
Herpesvirus entry mediator A antibody
Herpesvirus entry mediator antibody
Herpesvirus entry mediator ligand antibody
HveA antibody
HVEM antibody
ExpandImages
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☑ Cell treatment (CT),Relative expression (RE)
Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA722303) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: SK-MEL-28 cell lysate treated with deglycosylation
Lane 3: A549 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 40-50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722303) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH06-02] - BSA and Azide free
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH06-02]
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
