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HOXA13 Recombinant Rabbit Monoclonal Antibody [JE33-37]

Usd: 385

Specification

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Catalog# HA722305

HOXA13 Recombinant Rabbit Monoclonal Antibody [JE33-37]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

HOXA13 Recombinant Rabbit Monoclonal Antibody [JE33-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HOXA13 aa 250-350.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 40 kDa

Positive Control

293T cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse liver tissue.

Conjugation

unconjugated

Clone Number

JE33-37

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

Homeobox protein Hox-A13 is a protein that in humans is encoded by the HOXA13 gene. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation.

Background References

1. Qin Z et al. HOXA13 promotes gastric cancer progression partially via the FN1-mediated FAK/Src axis. Exp Hematol Oncol. 2022 Feb

2. Cui B et al. HOXA13 promotes liver regeneration through regulation of BMP-7. Biochem Biophys Res Commun. 2022 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P31271 Human

SwissProt: Q62424 Mouse

Entrez Gene: 500129 Rat

Synonyms

Homeo box 1J antibody

Homeo box A13 antibody

Homeobox 1J antibody

Homeobox A13 antibody

Homeobox protein Hox A13 antibody

Homeobox protein Hox-1J antibody

Homeobox protein Hox-A13 antibody

Homeobox protein HOXA13 antibody

Homeobox1J antibody

HomeoboxA13 antibody

Expand

Images

  • Western blot analysis of HOXA13 on different lysates with Rabbit anti-HOXA13 antibody (<a href="/products/HA722305" style="font-weight: bold;text-decoration: underline;">HA722305</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate (30 µg/Lane)<br />Lane 2: HepG2 cell lysate (30 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (30 µg/Lane)<br />Lane 4: C6 cell lysate (30 µg/Lane)<br /><br />Predicted band size: 40 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722305" style="font-weight: bold;text-decoration: underline;">HA722305</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HOXA13 on different lysates with Rabbit anti-HOXA13 antibody (HA722305) at 1/2,000 dilution.

    Lane 1: 293T cell lysate (30 µg/Lane)
    Lane 2: HepG2 cell lysate (30 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (30 µg/Lane)
    Lane 4: C6 cell lysate (30 µg/Lane)

    Predicted band size: 40 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HOXA13 antibody (<a href="/products/HA722305" style="font-weight: bold;text-decoration: underline;">HA722305</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722305" style="font-weight: bold;text-decoration: underline;">HA722305</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HOXA13 antibody (HA722305) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722305) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"