Retinoic Acid Receptor beta Recombinant Rabbit Monoclonal Antibody [JE36-74]
Catalog# HA722308
Retinoic Acid Receptor beta Recombinant Rabbit Monoclonal Antibody [JE36-74]
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WB
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Human
-
unconjugated
Overview
Product Name
Retinoic Acid Receptor beta Recombinant Rabbit Monoclonal Antibody [JE36-74]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Retinoic Acid Receptor beta aa 356-455 / 455.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 50 kDa
Positive Control
U-87 MG cell lysate, MCF7 cell lysate.
Conjugation
unconjugated
Clone Number
JE36-74
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Retinoic acid receptor beta (RAR-beta), also known as NR1B2 (nuclear receptor subfamily 1, group B, member 2) is a nuclear receptor that in humans is encoded by the RARB gene. This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. A deregulation of this gene has also been detected in uterine cervical carcinoma preneoplastic lesions. The gene expresses at least two transcript variants; one additional transcript has been described, but its full length nature has not been determined.
Background References
1. Yago-Ibáñez J et al. Retinoic acid receptor-beta prevents cisplatin-induced proximal tubular cell death. Biochim Biophys Acta Mol Basis Dis. 2020 Jul
2. Ciancia M et al. Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration. Prog Neurobiol. 2022 May
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
HAP antibody
HBV-activated protein antibody
NR1B2 antibody
Nuclear receptor subfamily 1 group B member 2 antibody
RAR B antibody
RAR beta antibody
RAR epsilon antibody
RAR-beta antibody
RAR-epsilon antibody
RARB antibody
ExpandImages
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Western blot analysis of Retinoic Acid Receptor beta on different lysates with Rabbit anti-Retinoic Acid Receptor beta antibody (HA722308) at 1/1,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722308) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"