PSAP Recombinant Rabbit Monoclonal Antibody [JE33-54]
Catalog# HA722322
PSAP Recombinant Rabbit Monoclonal Antibody [JE33-54]
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WB
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Human
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unconjugated
Overview
Product Name
PSAP Recombinant Rabbit Monoclonal Antibody [JE33-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human PSAP aa 1-150 / 524.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 58 kDa
Positive Control
HepG2 cell lysate, U-2 OS cell lysate, U-87 MG cell lysate, A549 cell lysate, SK-Br-3 cell lysate, LNCaP cell lysate, MCF7 cell lysate.
Conjugation
unconjugated
Clone Number
JE33-54
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Prosaposin, also known as PSAP, is a protein which in humans is encoded by the PSAP gene. This highly conserved glycoprotein is a precursor for 4 cleavage products: saposins A, B, C, and D. Saposin is an acronym for Sphingolipid Activator PrO[S]teINs. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.
Background References
1. Lin ZH et al. PSAP intronic variants around saposin D domain and Parkinson\'s disease. Brain. 2021 Feb
2. Zhao YW et al. PSAP variants in Parkinson\'s disease: a large cohort study in Chinese mainland population. Brain. 2021 Apr
Subcellular Location
Lysosome; Secreted.
UNIPROT
Synonyms
A1 activator antibody
Cerebroside sulfate activator antibody
Co-beta-glucosidase antibody
Component C antibody
CSAct antibody
Dispersin antibody
GLBA antibody
Glucosylceramidase activator antibody
Proactivator polypeptide antibody
Proactivator polypeptide precursor antibody
ExpandImages
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Western blot analysis of PSAP on different lysates with Rabbit anti-PSAP antibody (HA722322) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: A549 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: LNCaP cell lysate
Lane 7: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 80 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722322) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"