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PDE4B Recombinant Rabbit Monoclonal Antibody [JE33-95]

Usd: 385

Specification

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Catalog# HA722323

PDE4B Recombinant Rabbit Monoclonal Antibody [JE33-95]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

PDE4B Recombinant Rabbit Monoclonal Antibody [JE33-95]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant fragment within Human PDE4B aa 550 to the C-terminus.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 83 kDa

Positive Control

A549 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, mouse brain tissue, A549.

Conjugation

unconjugated

Clone Number

JE33-95

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

cAMP-specific 3',5'-cyclic phosphodiesterase 4B is an enzyme that in humans is encoded by the PDE4B gene. This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Background References

1. Wan Q et al. Targeting PDE4B (Phosphodiesterase-4 Subtype B) for Cardioprotection in Acute Myocardial Infarction via Neutrophils and Microcirculation. Circ Res. 2022 Aug

2. Herrmann FE et al. BI 1015550 is a PDE4B Inhibitor and a Clinical Drug Candidate for the Oral Treatment of Idiopathic Pulmonary Fibrosis. Front Pharmacol. 2022 Apr

Subcellular Location

Cytoplasm, Cell membrane.

UNIPROT

SwissProt: Q07343 Human

Entrez Gene: 18578 Mouse

Synonyms

5'-cyclic phosphodiesterase 4B antibody

cAMP-specific 3' antibody

cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody

cAMP-specific phosphodiesterase-4 B isoform antibody

DKFZp686F2182 antibody

DPDE4 antibody

dunce-like phosphodiesterase E4 antibody

MGC126529 antibody

OTTHUMP00000010652 antibody

OTTHUMP00000010653 antibody

Expand

Images

  • Western blot analysis of PDE4B on different lysates with Rabbit anti-PDE4B antibody (<a href="/products/HA722323" style="font-weight: bold;text-decoration: underline;">HA722323</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: Human brain tissue lysate (40 µg/Lane)<br />Lane 3: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 4: Mouse heart tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 83 kDa<br />Observed band size: 83 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722323" style="font-weight: bold;text-decoration: underline;">HA722323</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PDE4B on different lysates with Rabbit anti-PDE4B antibody (HA722323) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: Human brain tissue lysate (40 µg/Lane)
    Lane 3: Mouse brain tissue lysate (40 µg/Lane)
    Lane 4: Mouse heart tissue lysate (40 µg/Lane)

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE4B antibody (<a href="/products/HA722323" style="font-weight: bold;text-decoration: underline;">HA722323</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722323" style="font-weight: bold;text-decoration: underline;">HA722323</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE4B antibody (HA722323) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722323) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of A549 cells labeling PDE4B.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722323" style="font-weight: bold;text-decoration: underline;">HA722323</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A549 cells labeling PDE4B.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722323, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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