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KLF10 Recombinant Rabbit Monoclonal Antibody [JE34-09]

Usd: 385

Specification

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Catalog# HA722324

KLF10 Recombinant Rabbit Monoclonal Antibody [JE34-09]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

KLF10 Recombinant Rabbit Monoclonal Antibody [JE34-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human KLF10 aa 1-100 / 480.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 53 kDa

Positive Control

HepG2 cell lysate, Jurkat cell lysate, K-562 cell lysate, mouse lung tissue lysate, mouse testis tissue lysate, rat thymus tissue lysate.

Conjugation

unconjugated

Clone Number

JE34-09

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.

Background References

1. Yang S et al. KLF10 promotes nonalcoholic steatohepatitis progression through transcriptional activation of zDHHC7. EMBO Rep. 2022 Jun

2. Lin YM et al. KLF10 Functions as an Independent Prognosis Factor for Gastric Cancer. Medicina (Kaunas). 2022 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q13118 Human

SwissProt: O89091 Mouse

SwissProt: O08876 Rat

Synonyms

Early growth response, alpha antibody

Early growth response, alpha like antibody

EGR A antibody

EGR alpha antibody

EGR(A) antibody

EGR-alpha antibody

EGRA antibody

Egral antibody

EGRalpha antibody

Gdnfif antibody

Expand

Images

  • Western blot analysis of KLF10 on different lysates with Rabbit anti-KLF10 antibody (<a href="/products/HA722324" style="font-weight: bold;text-decoration: underline;">HA722324</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: K-562 cell lysate (20 µg/Lane)<br />Lane 4: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 5: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 6: Rat thymus tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 53 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722324" style="font-weight: bold;text-decoration: underline;">HA722324</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KLF10 on different lysates with Rabbit anti-KLF10 antibody (HA722324) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: K-562 cell lysate (20 µg/Lane)
    Lane 4: Mouse lung tissue lysate (40 µg/Lane)
    Lane 5: Mouse testis tissue lysate (40 µg/Lane)
    Lane 6: Rat thymus tissue lysate (40 µg/Lane)

    Predicted band size: 53 kDa
    Observed band size: 55 kDa

    Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"