Neuronatin Recombinant Rabbit Monoclonal Antibody [JE35-00]
Catalog# HA722326
Neuronatin Recombinant Rabbit Monoclonal Antibody [JE35-00]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Neuronatin Recombinant Rabbit Monoclonal Antibody [JE35-00]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Neuronatin aa 32-81 / 81.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 9 kDa
Positive Control
SH-SY5Y cell lysate, E14.0 mouse brain tissue lysate, mouse embryo tissue lysate, rat embryo tissue lysate, human brain tissue.
Conjugation
unconjugated
Clone Number
JE35-00
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
Target
Function
Neuronatin is a protein that in humans is encoded by the NNAT gene involved in mammalian brain development. It is located on Chromosome 20 in humans and is only expressed from the paternal allele in normal adults. The protein neuronatin, a proteolipid, that functions in the control of ion channels during brain development. Neuronatin begins the differentiation of pluripotent stem cells into cells with a neural fate by increasing their calcium levels. Neuronatin expression in neural tissues throughout the brain contributes to development of the nervous system. It is also expressed in several tissues outside of the brain. For example, expression in skin cells controls the differentiation of keratinocytes. Neuronatin expression functions not only in development, but other processes throughout the body.
Background References
1. Rudolph A et al. Circulating Neuronatin Levels Are Positively Associated with BMI and Body Fat Mass but Not with Psychological Parameters. Nutrients. 2023 Aug
2. Xing P et al. Neuronatin gene expression levels affect foetal growth and development by regulating glucose transport in porcine placenta. Gene. 2022 Jan
Subcellular Location
Cytoplasm.
Synonyms
Neuronatin antibody
NNAT antibody
NNAT_HUMAN antibody
Peg 5 antibody
Peg5 antibody
Images
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☑ Relative expression (RE)
Western blot analysis of Neuronatin on different lysates with Rabbit anti-Neuronatin antibody (HA722326) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SK-Br-3 cell lysate (negative)
Lane 3: E14.0 mouse brain tissue lysate
Lane 4: Mouse embryo tissue lysate
Lane 5: Rat embryo tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722326) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Neuronatin antibody (HA722326) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722326) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"