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Mov10 Recombinant Rabbit Monoclonal Antibody [JE35-40]

Usd: 385

Specification

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Catalog# HA722328

Mov10 Recombinant Rabbit Monoclonal Antibody [JE35-40]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Mov10 Recombinant Rabbit Monoclonal Antibody [JE35-40]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Mov10 aa 841-890 / 1,003.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 114 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, rat liver tissue lysate, rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

JE35-40

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Putative helicase MOV-10 is an enzyme that in humans is encoded by the MOV10 gene. Stability of MOV10 protein is controlled via DCAF12 ubiquitin ligase.

Background References

1. Nawaz A et al. Unwinding the roles of RNA helicase MOV10. Wiley Interdiscip Rev RNA. 2022 Mar

2. Wang L et al. MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity. mBio. 2021 Oct

Subcellular Location

Cytoplasm, P-body, Cytoplasmic ribonucleoprotein granule, Stress granule, Nucleus.

UNIPROT

SwissProt: Q9HCE1 Human

SwissProt: P23249 Mouse

Entrez Gene: 310756 Rat

Synonyms

DKFZp667O1423 antibody

FLJ32791 antibody

fSAP113 antibody

Functional spliceosome-associated protein 113 antibody

gb110 antibody

MGC2948 antibody

Moloney leukemia virus 10 protein antibody

MOV 10 antibody

Mov10 (Moloney leukemia virus 10, mouse) homolog antibody

Mov10 (Moloney leukemia virus 10,) homolog antibody

Expand

Images

  • Western blot analysis of Mov10 on different lysates with Rabbit anti-Mov10 antibody (<a href="/products/HA722328" style="font-weight: bold;text-decoration: underline;">HA722328</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: HepG2 cell lysate (20 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 5: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 6: C6 cell lysate (20 µg/Lane)<br />Lane 6: Rat liver tissue lysate (40 µg/Lane)<br />Lane 6: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 114 kDa<br />Observed band size: 114 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722328" style="font-weight: bold;text-decoration: underline;">HA722328</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Mov10 on different lysates with Rabbit anti-Mov10 antibody (HA722328) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: HepG2 cell lysate (20 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 5: RAW264.7 cell lysate (20 µg/Lane)
    Lane 6: C6 cell lysate (20 µg/Lane)
    Lane 6: Rat liver tissue lysate (40 µg/Lane)
    Lane 6: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722328) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"