HLTF Recombinant Rabbit Monoclonal Antibody [JE35-59]
Catalog# HA722329
HLTF Recombinant Rabbit Monoclonal Antibody [JE35-59]
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
HLTF Recombinant Rabbit Monoclonal Antibody [JE35-59]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human HLTF aa 910-1,009 / 1,009.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 114 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, HCT 116 cell lysate, HeLa, human ovary cancer tissue, human testis tissue.
Conjugation
unconjugated
Clone Number
JE35-59
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
-
1:100
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IHC-P
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1:200-1:1,000
Target
Function
Helicase-like transcription factor is an enzyme that in humans is encoded by the HLTF gene. This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. HLTF is a double-stranded DNA translocase, one of two human homologs of Saccharomyces cerevisiae RAD5 besides SHPRH (SNF2 histone linker PHD RING helicase), that is able to carry out fork regression, similarly to Rad5.
Background References
1. Xu Y et al. HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway. Oncogenesis. 2023 Jan
2. Bai G et al. HLTF Prevents G4 Accumulation and Promotes G4-induced Fork Slowing to Maintain Genome Stability. bioRxiv [Preprint]. 2023 Oct
Subcellular Location
Cytoplasm, Nucleus, nucleolus, nucleoplasm.
UNIPROT
Synonyms
DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
Helicase like transcription factor antibody
Helicase-like transcription factor antibody
HIP116 antibody
HIP116A antibody
HLTF 1 antibody
Hltf antibody
HLTF_HUMAN antibody
HLTF1 antibody
p113 antibody
ExpandImages
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Western blot analysis of HLTF on different lysates with Rabbit anti-HLTF antibody (HA722329) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HCT 116 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722329) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling HLTF with Rabbit anti-HLTF antibody (HA722329) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HLTF antibody (HA722329) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-HLTF antibody (HA722329) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722329) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-HLTF antibody (HA722329) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722329) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"