SLC22A1 Recombinant Rabbit Monoclonal Antibody [JE37-67]
Catalog# HA722335
SLC22A1 Recombinant Rabbit Monoclonal Antibody [JE37-67]
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WB
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Human
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Green monkey
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unconjugated
Overview
Product Name
SLC22A1 Recombinant Rabbit Monoclonal Antibody [JE37-67]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SLC22A1 aa 81-180 / 554.
Species Reactivity
Human, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 61 kDa
Positive Control
SW480 cell lysate, HepG2 cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
JE37-67
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Solute carrier family 22 member 1 is a protein that in humans is encoded by the gene SLC22A1. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. It is also required for the uptake of metformin by cells.
Background References
1. Yee SW et al. The full spectrum of OCT1 (SLC22A1) mutations bridges transporter biophysics to drug pharmacogenomics. bioRxiv [Preprint]. 2023 Jun
2. Cahua-Pablo JÁ et al. Genetic variants in SLC22A1 are related to serum lipid levels in Mexican women. Lipids. 2022 Mar
Subcellular Location
Basolateral cell membrane, Apical cell membrane, Lateral cell membrane, Basal cell membrane, Cell membrane.
UNIPROT
Synonyms
hOCT1 antibody
OCT1 antibody
oct1_cds antibody
Organic cation transporter 1 antibody
S22A1_HUMAN antibody
Slc22a1 antibody
solute carrier family 22 (organic cation transporter), member 1 antibody
Solute carrier family 22 member 1 antibody
Images
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Western blot analysis of SLC22A1 on different lysates with Rabbit anti-SLC22A1 antibody (HA722335) at 1/1,000 dilution.
Lane 1: SW480 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 70 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722335) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"