FLAP Recombinant Rabbit Monoclonal Antibody [JE39-03]
Catalog# HA722341
FLAP Recombinant Rabbit Monoclonal Antibody [JE39-03]
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WB
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Human
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unconjugated
Overview
Product Name
FLAP Recombinant Rabbit Monoclonal Antibody [JE39-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human FLAP aa 100-161.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 18 kDa
Positive Control
U-937 cell lysate, THP-1 cell lysate, THP-1 treated with 50nM TPA overnight then add 5μg/mL LPS for 8 hours cell lysate.
Conjugation
unconjugated
Clone Number
JE39-03
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Arachidonate 5-lipoxygenase-activating protein also known as 5-lipoxygenase activating protein, or FLAP, is a protein that in humans is encoded by the ALOX5AP gene. FLAP is necessary for the activation of 5-lipoxygenase and therefore for the production of leukotrienes, 5-hydroxyeicosatetraenoic acid, 5-oxo-eicosatetraenoic acid, and specialized pro-resolving mediators of the lipoxin and resolvin classes. It is an integral protein within the nuclear membrane. FLAP is necessary in synthesis of leukotriene, which are lipid mediators of inflammation that is involved in respiratory and cardiovascular diseases. FLAP functions as a membrane anchor for 5-lipooxygenase and as an amine acid-bind protein. How FLAP activates 5-lipooxygenase is not completely understood, but there is a physical interaction between the two. FLAP structure consists of 4 transmembrane alpha helices, but they are found in trimer forming a barrel. The barrel is about 60 Å high and 36 Å wide.
Background References
1. Chen XY et al. ALOX5AP is a new prognostic indicator in acute myeloid leukemia. Discov Oncol. 2023 Nov
2. Ye X et al. ALOX5AP Predicts Poor Prognosis by Enhancing M2 Macrophages Polarization and Immunosuppression in Serous Ovarian Cancer Microenvironment. Front Oncol. 2021 May
Subcellular Location
Nucleus membrane, Endoplasmic reticulum membrane.
UNIPROT
Synonyms
5-lipoxygenase activating protein antibody
AL5AP_HUMAN antibody
ALOX 5AP antibody
ALOX5 AP antibody
ALOX5AP antibody
Arachidonate 5 lipoxygenase activating protein antibody
Arachidonate 5-lipoxygenase-activating protein antibody
Five lipoxygenase activating protein antibody
FLAP antibody
MK 886 binding protein antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of FLAP on different lysates with Rabbit anti-FLAP antibody (HA722341) at 1/1,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: THP-1 treated with 50nM TPA overnight then add 5μg/mL LPS for 8 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722341) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"