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MSP / MST1 Recombinant Rabbit Monoclonal Antibody [JE39-26]

Usd: 385

Specification

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Catalog# HA722342

MSP / MST1 Recombinant Rabbit Monoclonal Antibody [JE39-26]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MSP / MST1 Recombinant Rabbit Monoclonal Antibody [JE39-26]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human MST1 aa 19-68.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB

Molecular Weight

Predicted band size: 80 kDa

Positive Control

HeLa cell lysate, PC-3 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, rat spleen tissue lysate.

Conjugation

unconjugated

Clone Number

JE39-26

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Macrophage-stimulating protein (MSP), also known as hepatocyte growth factor-like protein (HLP, HGFL, or HGFLP), is a protein that in humans is encoded by the MST1 (macrophage-stimulating 1) gene.

Background References

1. Quan M et al. MST1 Suppresses Disturbed Flow Induced Atherosclerosis. Circ Res. 2022 Oct

2. Shao Y et al. MST1: A future novel target for cardiac diseases. Int J Biol Macromol. 2023 Jun

Subcellular Location

Secreted.

UNIPROT

SwissProt: P26927 Human

SwissProt: P26928 Mouse

Entrez Gene: 24566 Rat

Synonyms

D3F15S2 antibody

DNF15S2 antibody

Hepatocyte growth factor like protein alpha chain antibody

Hepatocyte growth factor like protein antibody

Hepatocyte growth factor like protein beta chain antibody

Hepatocyte growth factor like protein homolog antibody

Hepatocyte growth factor-like protein beta chain antibody

HGFL antibody

HGFL_HUMAN antibody

Macrophage stimulating 1 (hepatocyte growth factor like) antibody

Expand

Images

  • Western blot analysis of MSP / MST1 on different lysates with Rabbit anti-MSP / MST1 antibody (<a href="/products/HA722342" style="font-weight: bold;text-decoration: underline;">HA722342</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: PC-3 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: C2C12 cell lysate (20 µg/Lane)<br />Lane 5: C6 cell lysate (20 µg/Lane)<br />Lane 6: COS-1 cell lysate (20 µg/Lane)<br />Lane 7: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 80 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722342" style="font-weight: bold;text-decoration: underline;">HA722342</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MSP / MST1 on different lysates with Rabbit anti-MSP / MST1 antibody (HA722342) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: PC-3 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: C2C12 cell lysate (20 µg/Lane)
    Lane 5: C6 cell lysate (20 µg/Lane)
    Lane 6: COS-1 cell lysate (20 µg/Lane)
    Lane 7: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 80 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722342) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation