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ZNF217 Recombinant Rabbit Monoclonal Antibody [JE39-40]

Usd: 385

Specification

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Catalog# HA722344

ZNF217 Recombinant Rabbit Monoclonal Antibody [JE39-40]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ZNF217 Recombinant Rabbit Monoclonal Antibody [JE39-40]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ZNF217 aa 1000-1048.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 115 kDa

Positive Control

MCF7 cell lysate, PANC-1 cell lysate, HEK-293 cell lysate.

Conjugation

unconjugated

Clone Number

JE39-40

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Zinc finger protein 217, also known as ZNF217, is a protein which in humans is encoded by the ZNF217 gene. ZNF217 can attenuate apoptotic signals resulting from telomere dysfunction and may promote neoplastic transformation and later stages of malignancy. Znf217 was shown to be a prognostic biomarker and therapeutic target during breast cancer progression.

Background References

1. Fahmé P et al. The Intricate Interplay between the ZNF217 Oncogene and Epigenetic Processes Shapes Tumor Progression. Cancers (Basel). 2022 Dec

2. Li Y et al. ZNF217: the cerberus who fails to guard the gateway to lethal malignancy. Am J Cancer Res. 2021 Jul

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O75362 Human

Synonyms

ZABC 1 antibody

ZABC1 antibody

Zinc finger protein 217 antibody

ZN 217 antibody

ZN217 antibody

ZN217_HUMAN antibody

ZNF 217 antibody

ZNF217 antibody

Images

  • Western blot analysis of ZNF217 on different lysates with Rabbit anti-ZNF217 antibody (<a href="/products/HA722344" style="font-weight: bold;text-decoration: underline;">HA722344</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: PANC-1 cell lysate<br />Lane 3: HEK-293 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 115 kDa<br />Observed band size: 140 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722344" style="font-weight: bold;text-decoration: underline;">HA722344</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ZNF217 on different lysates with Rabbit anti-ZNF217 antibody (HA722344) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: PANC-1 cell lysate
    Lane 3: HEK-293 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 115 kDa
    Observed band size: 140 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722344) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"