Phospho-MAP2 (S136) Recombinant Rabbit Monoclonal Antibody [JE44-25]
Catalog# HA722351
Phospho-MAP2 (S136) Recombinant Rabbit Monoclonal Antibody [JE44-25]
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Mouse
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Rat
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unconjugated
Overview
Product Name
Phospho-MAP2 (S136) Recombinant Rabbit Monoclonal Antibody [JE44-25]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser136 of human MAP2.
Product Specificity
Phospho-MAP2 (Ser136) (JE44-25) Antibody detects endogenous levels of all isoforms of MAP2 only when phosphorylated at serine 136.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P, IHC-Fr, IF-Tissue
Molecular Weight
Predicted band size: 200 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
JE44-25
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IHC-P
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1:1,000
-
IHC-Fr
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1:100
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IF-Tissue
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1:200
Target
Function
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family were originally isolated since they copurify with tubulin in polymerization experiments: tubulin in cell extracts can be made to polymerize to produce microtubules (MT) under the influence of heat and the addition of GTP, and the MT can then be collected by centrifugation. When this is done a series of microtubule associated proteins are collected along with the MT and can be detected by SDS-PAGE and other methods. Brain extracts are rich in several of these proteins, MAP2 being one of these. The single MAP2 gene produces four major transcripts producing four proteins, MAP2A, MAP2B, MAP2C and MAP2D. MAP2A and MAP2B are very high molecular weight proteins, with apparent molecular weight on SDS-PAGE about 250kDa, while MAP2C and MAP2D are much lower molecular weight forms with apparent SDS-PAGE size about 70kDa. All forms of MAP2 share a common core sequence which includes MT binding domains, 18 amino acid sequences which are found in other MT associated proteins such as MAP Tau and MAP1B. The MAP2 isoforms are thought to be involved in MT assembly, which is an essential step in neuritogenesis. MAP2 serves to stabilize MT growth by crosslinking MT with intermediate filaments and other MTs. MAP2 isoforms are neuron-specific cytoskeletal proteins enriched in dendrites and perikarya, implicating a role in determining and stabilizing neuronal morphology during neuron development. As a result antibodies to MAP2 are widely used to identify neuronal cells and trace dendritic processes in experimental contexts.
Background References
1. Holden MR et al. MAP2 caps tau fibrils and inhibits aggregation. J Biol Chem. 2023 Jul
2. Grubisha MJ et al. MAP2 is differentially phosphorylated in schizophrenia, altering its function. Mol Psychiatry. 2021 Sep
Subcellular Location
Cytoplasm, cytoskeleton, Cell projection, dendrite.
Synonyms
DKFZp686I2148 antibody
MAP 2 antibody
MAP dendrite specific antibody
MAP-2 antibody
MAP2 antibody
MAP2A antibody
MAP2B antibody
MAP2C antibody
Microtubule associated protein 2 antibody
Microtubule-associated protein 2 antibody
ExpandImages
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Western blot analysis of Phospho-MAP2 (S136) on different lysates with Rabbit anti-Phospho-MAP2 (S136) antibody (HA722351) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 200 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722351) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded human brain tissue untreated / treated with λpp with Rabbit anti-Phospho-MAP2 (S136) antibody (HA722351) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722351) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue untreated / treated with λpp with Rabbit anti-Phospho-MAP2 (S136) antibody (HA722351) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722351) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded rat brain tissue untreated / treated with λpp with Rabbit anti-Phospho-MAP2 (S136) antibody (HA722351) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722351) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Phospho-MAP2 (S136) antibody (HA722351) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722351, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"