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DCAF13 Recombinant Rabbit Monoclonal Antibody [JE46-31]

Usd: 385

Specification

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Catalog# HA722352

DCAF13 Recombinant Rabbit Monoclonal Antibody [JE46-31]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

DCAF13 Recombinant Rabbit Monoclonal Antibody [JE46-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DCAF13 aa 1-100 / 445.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 51 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse colon tissue, mouse ovary tissue, rat ovary tissue.

Conjugation

unconjugated

Clone Number

JE46-31

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • IP

  • 1-2μg/sample

Target

Function

Enables estrogen receptor binding activity. Predicted to be involved in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA). Located in several cellular components, including centrosome; cytosol; and nuclear lumen. Part of Cul4-RING E3 ubiquitin ligase complex. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Participates in the 18S rRNA processing in growing oocytes, being essential for oocyte nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) transition.

Background References

1. Shan BQ et al. DCAF13 promotes breast cancer cell proliferation by ubiquitin inhibiting PERP expression. Cancer Sci. 2022 May

2. Wei S et al. A multidimensional pan-cancer analysis of DCAF13 and its protumorigenic effect in lung adenocarcinoma. FASEB J. 2023 Apr

Subcellular Location

Nucleus, nucleolus.

UNIPROT

SwissProt: Q9NV06 Human

SwissProt: Q6PAC3 Mouse

Entrez Gene: 362902 Rat

Synonyms

DCA13_HUMAN antibody

DCAF13 antibody

DDB1 and CUL4 associated factor 13 antibody

DDB1- and CUL4-associated factor 13 antibody

DKFZP564O0463 antibody

GM83 antibody

HSPC064 antibody

MGC126859 antibody

MGC138247 antibody

WD repeat and SOF domain-containing protein 1 antibody

Expand

Images

  • Western blot analysis of DCAF13 on different lysates with Rabbit anti-DCAF13 antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 5: PC-12 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 51 kDa<br />Observed band size: 49 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DCAF13 on different lysates with Rabbit anti-DCAF13 antibody (HA722352) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: Neuro-2a cell lysate (20 µg/Lane)
    Lane 5: PC-12 cell lysate (20 µg/Lane)

    Predicted band size: 51 kDa
    Observed band size: 49 kDa

    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722352) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-DCAF13 antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-DCAF13 antibody (HA722352) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722352) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-DCAF13 antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-DCAF13 antibody (HA722352) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722352) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-DCAF13 antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-DCAF13 antibody (HA722352) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722352) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DCAF13 was immunoprecipitated from 0.2 mg 293T cell lysate with <a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: 293T cell lysate (input)<br />Lane 2: <a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a> IP in 293T cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722352" style="font-weight: bold;text-decoration: underline;">HA722352</a> in 293T cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 2 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    DCAF13 was immunoprecipitated from 0.2 mg 293T cell lysate with HA722352 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722352 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: 293T cell lysate (input)
    Lane 2: HA722352 IP in 293T cell lysate
    Lane 3: Rabbit IgG instead of HA722352 in 293T cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 2 minutes; ECL: K1801

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