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RAIDD Recombinant Rabbit Monoclonal Antibody [JE47-11]

Usd: 385

Specification

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Catalog# HA722353

RAIDD Recombinant Rabbit Monoclonal Antibody [JE47-11]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RAIDD Recombinant Rabbit Monoclonal Antibody [JE47-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human RAIDD aa 100-199.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 23 kDa

Positive Control

Raji cell lysate, HeLa cell lysate, K-562 cell lysate, rat heart tissue lysate, rat testis tissue lysate, human heart tissue, human testis tissue.

Conjugation

unconjugated

Clone Number

JE47-11

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Death domain-containing protein CRADD is a protein that in humans is encoded by the CRADD gene. The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins.

Background References

1. Koprulu M et al. CRADD and USP44 mutations in intellectual disability, mild lissencephaly, brain atrophy, developmental delay, strabismus, behavioural problems and skeletal anomalies. Eur J Med Genet. 2021 Apr

2. Ha HJ et al. RAIDD mutations underlie the pathogenesis of thin lissencephaly (TLIS). PLoS One. 2018 Oct

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P78560 Human

Entrez Gene: 314756 Rat

Synonyms

CASP2 and RIPK1 domain containing adaptor with death domain antibody

Caspase and RIP adapter with death domain antibody

Caspase and RIP adaptor with death domain antibody

Cradd antibody

CRADD_HUMAN antibody

Death adaptor molecule RAIDD antibody

Death domain containing protein CRADD antibody

Death domain-containing protein CRADD antibody

MGC9163 antibody

RIP associated ICH1/CED3 homologous protein with death domain antibody

Expand

Images

  • Western blot analysis of RAIDD on different lysates with Rabbit anti-RAIDD antibody (<a href="/products/HA722353" style="font-weight: bold;text-decoration: underline;">HA722353</a>) at 1/1,000 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: Rat heart tissue lysate<br />Lane 5: Rat testis tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 23 kDa<br />Observed band size: 23 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722353" style="font-weight: bold;text-decoration: underline;">HA722353</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RAIDD on different lysates with Rabbit anti-RAIDD antibody (HA722353) at 1/1,000 dilution.

    Lane 1: Raji cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: Rat heart tissue lysate
    Lane 5: Rat testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722353) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-RAIDD antibody (<a href="/products/HA722353" style="font-weight: bold;text-decoration: underline;">HA722353</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722353" style="font-weight: bold;text-decoration: underline;">HA722353</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-RAIDD antibody (HA722353) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722353) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RAIDD antibody (<a href="/products/HA722353" style="font-weight: bold;text-decoration: underline;">HA722353</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722353" style="font-weight: bold;text-decoration: underline;">HA722353</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RAIDD antibody (HA722353) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722353) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"