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ENDOGL1 / ENGL Recombinant Rabbit Monoclonal Antibody [JE63-12]

Usd: 385

Specification

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Catalog# HA722356

ENDOGL1 / ENGL Recombinant Rabbit Monoclonal Antibody [JE63-12]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ENDOGL1 / ENGL Recombinant Rabbit Monoclonal Antibody [JE63-12]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ENDOGL1 / ENGL aa 341-390 / 368.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 41 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, HUVEC cell lysate, HL-60 cell lysate, MCF7 cell lysate, human heart tissue.

Conjugation

unconjugated

Clone Number

JE63-12

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18.

Background References

1. Szymanski MR et al. Human EXOG Possesses Strong AP Hydrolysis Activity: Implication on Mitochondrial DNA Base Excision Repair. J Am Chem Soc. 2022 Dec

2. Xiao J et al. PGC-1 Mediated-EXOG, a Specific Repair Enzyme for Mitochondrial DNA, Plays an Essential Role in the Rotenone-Induced Neurotoxicity of PC12 Cells. J Mol Neurosci. 2021 Nov

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: Q9Y2C4 Human

Synonyms

Endo G like 1 antibody

Endo G-like 1 antibody

Endo/exonuclease (5'-3'), endonuclease G like antibody

ENDOGL 1 antibody

ENDOGL1 antibody

ENDOGL2 antibody

Endonuclease G like 1 antibody

Endonuclease G like 2 antibody

Endonuclease G-like 1 antibody

ENGL a antibody

Expand

Images

  • Western blot analysis of ENDOGL1 / ENGL on different lysates with Rabbit anti-ENDOGL1 / ENGL antibody (<a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: HUVEC cell lysate<br />Lane 4: HL-60 cell lysate<br />Lane 5: MCF7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ENDOGL1 / ENGL on different lysates with Rabbit anti-ENDOGL1 / ENGL antibody (HA722356) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HUVEC cell lysate
    Lane 4: HL-60 cell lysate
    Lane 5: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722356) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-ENDOGL1 / ENGL antibody (<a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-ENDOGL1 / ENGL antibody (HA722356) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722356) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ENDOGL1 / ENGL was immunoprecipitated from 0.2 mg HUVEC cell lysate with <a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HUVEC cell lysate (input)<br />Lane 2: <a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a> IP in HUVEC cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722356" style="font-weight: bold;text-decoration: underline;">HA722356</a> in HUVEC cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 10 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    ENDOGL1 / ENGL was immunoprecipitated from 0.2 mg HUVEC cell lysate with HA722356 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722356 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HUVEC cell lysate (input)
    Lane 2: HA722356 IP in HUVEC cell lysate
    Lane 3: Rabbit IgG instead of HA722356 in HUVEC cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 10 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"