PEF1 Recombinant Rabbit Monoclonal Antibody [JE63-88]
Catalog# HA722358
PEF1 Recombinant Rabbit Monoclonal Antibody [JE63-88]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
PEF1 Recombinant Rabbit Monoclonal Antibody [JE63-88]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PEF1 aa 100-200.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 30 kDa
Positive Control
Jurkat cell lysate, HeLa cell lysate, 293T cell lysate, HL-60 cell lysate, rat pancreas tissue lysate.
Conjugation
unconjugated
Clone Number
JE63-88
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Peflin is a protein that in humans is encoded by the PEF1 gene. PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF hand (PEF) protein family, which includes the calpain small subunit, sorcin, grancalcin, and ALG2. PEF1 has been shown to interact with PDCD6.
Background References
1. Matsuda S et al. The S. pombe CDK5 Orthologue Pef1 Cooperates with Three Cyclins, Clg1, Pas1 and Psl1, to Promote Pre-Meiotic DNA Replication. Biomolecules. 2021 Jan
2. Birot A et al. The CDK Pef1 and protein phosphatase 4 oppose each other for regulating cohesin binding to fission yeast chromosomes. Elife. 2020 Jan
Subcellular Location
Cytoplasm, Endoplasmic reticulum, Membrane, Cytoplasmic vesicle, COPII-coated vesicle membrane.
Synonyms
ABP32 antibody
PEF antibody
PEF protein with a long N terminal hydrophobic domain antibody
PEF protein with a long N-terminal hydrophobic domain antibody
pef1 antibody
PEF1_HUMAN antibody
PEF1A antibody
Peflin antibody
Penta EF hand domain containing protein 1 antibody
Penta-EF hand domain-containing protein 1 antibody
Images
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Western blot analysis of PEF1 on different lysates with Rabbit anti-PEF1 antibody (HA722358) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HL-60 cell lysate (20 µg/Lane)
Lane 5: Rat pancreas tissue lysate (40 µg/Lane)
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: Lane 1-4: 20 seconds; Lane 5: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722358) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"