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TOR4A Recombinant Rabbit Monoclonal Antibody [JE64-50]

Usd: 385

Specification

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Catalog# HA722363

TOR4A Recombinant Rabbit Monoclonal Antibody [JE64-50]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TOR4A Recombinant Rabbit Monoclonal Antibody [JE64-50]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TOR4A aa 1-150 / 423.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 47 kDa

Positive Control

THP-1 cell lysate, K-562 cell lysate, HeLa cell lysate.

Conjugation

unconjugated

Clone Number

JE64-50

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Predicted to enable ATP binding activity. Predicted to be located in extracellular region and platelet alpha granule lumen. Predicted to be active in endoplasmic reticulum lumen and nuclear envelope.

Background References

1. Wang Q et al. Delta and Notch-like epidermal growth factor-related receptor suppresses human glioma growth by inhibiting oncogene TOR4A. J Cancer Res Ther. 2022 Sep

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q9NXH8 Human

Synonyms

Torsin-4A

Torsin family 4 member A

TOR4A

C9orf167

Images

  • Western blot analysis of TOR4A on different lysates with Rabbit anti-TOR4A antibody (<a href="/products/HA722363" style="font-weight: bold;text-decoration: underline;">HA722363</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 47 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722363" style="font-weight: bold;text-decoration: underline;">HA722363</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TOR4A on different lysates with Rabbit anti-TOR4A antibody (HA722363) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 47 kDa
    Observed band size: 45 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722363) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"