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SYPL1 Recombinant Rabbit Monoclonal Antibody [JE77-41]

Usd: 385

Specification

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Catalog# HA722387

SYPL1 Recombinant Rabbit Monoclonal Antibody [JE77-41]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

SYPL1 Recombinant Rabbit Monoclonal Antibody [JE77-41]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human SYPL1 aa 110-259 / 259.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 29 kDa

Positive Control

BxPC-3 cell lysate, PANC-1 cell lysate, human lung tissue lysate, human cerebellum tissue, human testis tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

JE77-41

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Predicted to be involved in chemical synaptic transmission. Located in extracellular exosome. Most malignant cells exhibited moderate cytoplasmic immunoreactivity. Malignant melanomas, urothelial cancers and a few cases of gastric adenocarcinomas demonstrated strong cytoplasmic positivity. Malignant gliomas, breast, prostate, testis and skin cancers displayed weak staining or negative cells in several cases.

Background References

1. Shu T., Wu K., Guo Y., He Q., Song X., Shan J., Wu L., Liu J., Wang Z., Sun X. Evaluation of fecal SYPL1 as a diagnostic biomarker in colorectal cancer. Clin Biochem 103:8-15 (2022)

2. Liu L., He Q., Li Y., Zhang B., Sun X., Shan J., Pan B., Zhang T., Zhao Z., Guo Y. Serum SYPL1 is a promising diagnostic biomarker for colorectal cancer. Clin Chim Acta 509:36-42 (2020)

Subcellular Location

Cytoplasmic vesicle membrane.

UNIPROT

SwissProt: Q16563 Human

SwissProt: O09117 Mouse

Synonyms

H-SP1 antibody

Pantophysin antibody

Synaptophysin-like protein 1 antibody

SYPL antibody

Sypl1 antibody

SYPL1_HUMAN antibody

Images

  • Western blot analysis of SYPL1 on different lysates with Rabbit anti-SYPL1 antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution.<br /><br />Lane 1: BxPC-3 cell lysate<br />Lane 2: PANC-1 cell lysate<br />Lane 3: Human lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29-37 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SYPL1 on different lysates with Rabbit anti-SYPL1 antibody (HA722387) at 1/1,000 dilution.

    Lane 1: BxPC-3 cell lysate
    Lane 2: PANC-1 cell lysate
    Lane 3: Human lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 29 kDa
    Observed band size: 29-37 kDa

    Exposure time: 25 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722387) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-SYPL1 antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-SYPL1 antibody (HA722387) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722387) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SYPL1 antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SYPL1 antibody (HA722387) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722387) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SYPL1 antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722387" style="font-weight: bold;text-decoration: underline;">HA722387</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SYPL1 antibody (HA722387) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722387) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"