Nogo Receptor Recombinant Rabbit Monoclonal Antibody [JE33-68]
Catalog# HA722391
Nogo Receptor Recombinant Rabbit Monoclonal Antibody [JE33-68]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Nogo Receptor Recombinant Rabbit Monoclonal Antibody [JE33-68]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Nogo Receptor aa 341-440 / 473.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 51 kDa
Positive Control
SH-SY5Y cell lysate, HepG2 cell lysate, HEK-293 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE33-68
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Reticulon 4 receptor (RTN4R) also known as Nogo-66 Receptor (NgR) or Nogo receptor 1 is a protein which in humans is encoded by the RTN4R gene. This gene encodes the receptor for reticulon 4, oligodendrocytemyelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. The Nogo-66 Receptor (NgR) is a high affinity binding receptor for a region of Nogo, a myelin associated protein that inhibits axon outgrowth. NgR was identified by Strittmatter and colleagues using an expression cloning strategy. NgR is implicated in neuronal plasticity and regeneration. Its relative importance in mediating myelin inhibition in vitro and in vivo is currently under intense investigation, since this protein might be a good drug target for treatment of various neurological conditions such as spinal cord injury and stroke.
Background References
1. Wang J et al. RTN4/NoGo-receptor binding to BAI adhesion-GPCRs regulates neuronal development. Cell. 2021 Nov
2. Rodriguez CM et al. Targeting RTN4/NoGo-Receptor reduces levels of ALS protein ataxin-2. Cell Rep. 2022 Oct
Subcellular Location
Cell membrane, Membrane raft, Cell projection, dendrite, axon, Perikaryon.
Synonyms
NgR antibody
Nogo 66 receptor antibody
Nogo receptor antibody
Nogor antibody
Reticulon 4 receptor antibody
Rtn4r antibody
UNQ330/PRO526 antibody
Images
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Western blot analysis of Nogo Receptor on different lysates with Rabbit anti-Nogo Receptor antibody (HA722391) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (30 µg/Lane)
Lane 6: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 51 kDa
Observed band size: 65 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"