DSTN Recombinant Rabbit Monoclonal Antibody [JE36-06]
Catalog# HA722397
DSTN Recombinant Rabbit Monoclonal Antibody [JE36-06]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
DSTN Recombinant Rabbit Monoclonal Antibody [JE36-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human DSTN aa 1-100 / 165.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 19 kDa
Positive Control
HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human brain tissue.
Conjugation
unconjugated
Clone Number
JE36-06
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Destrin or DSTN (also known as actin depolymerizing factor or ADF) is a protein which in humans is encoded by the DSTN gene. Destrin is a component protein in microfilaments. The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene.
Background References
1. Wen R et al. DSTN Hypomethylation Promotes Radiotherapy Resistance of Rectal Cancer by Activating the Wnt/beta-Catenin Signaling Pathway. Int J Radiat Oncol Biol Phys. 2023 Sep
2. Li Y et al. Acquired MET-DSTN Fusion Mediated Resistance to EGFR-TKIs in Lung Adenocarcinoma and Responded to Crizotinib Plus Gefitinib: A Case Report. Clin Lung Cancer. 2022 Jan
Subcellular Location
Actin cytoskeleton, cortical actin cytoskeleton, cytoplasm, extracellular exosome.
Synonyms
2610043P17Rik antibody
ACTDP antibody
Actin depolymerizing factor antibody
Actin-depolymerizing factor antibody
ADF antibody
AU042046 antibody
bA462D18.2 antibody
corn1 antibody
DEST_HUMAN antibody
Destrin (actin depolymerizing factor) antibody
ExpandImages
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Western blot analysis of DSTN on different lysates with Rabbit anti-DSTN antibody (HA722397) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: A549 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722397) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DSTN antibody (HA722397) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722397) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"