Skip to content

DSTN Recombinant Rabbit Monoclonal Antibody [JE36-06]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA722397

DSTN Recombinant Rabbit Monoclonal Antibody [JE36-06]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

DSTN Recombinant Rabbit Monoclonal Antibody [JE36-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DSTN aa 1-100 / 165.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 19 kDa

Positive Control

HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human brain tissue.

Conjugation

unconjugated

Clone Number

JE36-06

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Destrin or DSTN (also known as actin depolymerizing factor or ADF) is a protein which in humans is encoded by the DSTN gene. Destrin is a component protein in microfilaments. The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene.

Background References

1. Wen R et al. DSTN Hypomethylation Promotes Radiotherapy Resistance of Rectal Cancer by Activating the Wnt/beta-Catenin Signaling Pathway. Int J Radiat Oncol Biol Phys. 2023 Sep

2. Li Y et al. Acquired MET-DSTN Fusion Mediated Resistance to EGFR-TKIs in Lung Adenocarcinoma and Responded to Crizotinib Plus Gefitinib: A Case Report. Clin Lung Cancer. 2022 Jan

Subcellular Location

Actin cytoskeleton, cortical actin cytoskeleton, cytoplasm, extracellular exosome.

UNIPROT

SwissProt: P60981 Human

SwissProt: Q9R0P5 Mouse

SwissProt: Q7M0E3 Rat

Synonyms

2610043P17Rik antibody

ACTDP antibody

Actin depolymerizing factor antibody

Actin-depolymerizing factor antibody

ADF antibody

AU042046 antibody

bA462D18.2 antibody

corn1 antibody

DEST_HUMAN antibody

Destrin (actin depolymerizing factor) antibody

Expand

Images

  • Western blot analysis of DSTN on different lysates with Rabbit anti-DSTN antibody (<a href="/products/HA722397" style="font-weight: bold;text-decoration: underline;">HA722397</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 19 kDa<br />Observed band size: 19 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722397" style="font-weight: bold;text-decoration: underline;">HA722397</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DSTN on different lysates with Rabbit anti-DSTN antibody (HA722397) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722397) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DSTN antibody (<a href="/products/HA722397" style="font-weight: bold;text-decoration: underline;">HA722397</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722397" style="font-weight: bold;text-decoration: underline;">HA722397</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DSTN antibody (HA722397) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722397) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"