HOXA5 Recombinant Rabbit Monoclonal Antibody [JE36-98]
Catalog# HA722398
HOXA5 Recombinant Rabbit Monoclonal Antibody [JE36-98]
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WB
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Human
-
Rat
-
unconjugated
Overview
Product Name
HOXA5 Recombinant Rabbit Monoclonal Antibody [JE36-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HOXA5 aa 100-200.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 29 kDa
Positive Control
293T cell lysate, SH-SY5Y cell lysate, Caco-2 cell lysate, rat heart tissue lysate, rat kidney tissue lysate.
Conjugation
unconjugated
Clone Number
JE36-98
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Homeobox protein Hox-A5 is a protein that in humans is encoded by the HOXA5 gene. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis.
Background References
1. Zhang Y et al. HINT1 (Histidine Triad Nucleotide-Binding Protein 1) Attenuates Cardiac Hypertrophy Via Suppressing HOXA5 (Homeobox A5) Expression. Circulation. 2021 Aug
2. Parrillo L et al. The Transcription Factor HOXA5: Novel Insights into Metabolic Diseases and Adipose Tissue Dysfunction. Cells. 2023 Aug
Subcellular Location
Nucleus.
Synonyms
Homeo box 1C antibody
Homeo box A5 antibody
Homeobox A5 antibody
Homeobox protein Hox-1C antibody
Homeobox protein Hox-A5 antibody
Homeobox protein HOXA5 antibody
HOX 1 antibody
HOX 1.3 antibody
HOX 1C antibody
HOX1 antibody
ExpandImages
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Western blot analysis of HOXA5 on different lysates with Rabbit anti-HOXA5 antibody (HA722398) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: Caco-2 cell lysate (20 µg/Lane)
Lane 4: Rat heart tissue lysate (40 µg/Lane)
Lane 5: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722398) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"